Frederick Sanger

A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.

Research Article| January 01 1945 The free amino groups of insulin F. Sanger F. Sanger 1Biochemical Laboratory, Cambridge Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1945) 39 (5): 507–515. https://doi.org/10.1042/bj0390507 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation F. Sanger; The free amino groups of insulin. Biochem J 1 January 1945; 39 (5): 507–515. doi: https://doi.org/10.1042/bj0390507 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1945 CAMBRIDGE UNIVERSITY PRESS, ENGLAND1945 Article PDF first page preview Close Modal You do not currently have access to this content.