Cited by 90
University of California, Davis
Publishes on Viral-associated cancers and disorders, Cytomegalovirus and herpesvirus research, Reproductive System and Pregnancy. 6 papers and 203 citations.
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The leukocyte-transforming agent was found in the pharyngeal secretions of 75% of 36 patients with infectious mononucleosis (1M), 18% of 27 persons with acute illness other than 1M, and 27% of 11 healthy persons. Five of 12 patients with 1M were still excreting the agent three to 12 months after onset of illness. When the pharyngeal secretion was tested at intervals throughout the course of illness instead of only once at the time of admission, all five patients with 1M whose first throat washing was negative were shown to excrete the transforming agent at sometime in the course of illness.
A viral etiology for infectious mononucleosis (IM) has been suspected for many years. There is recent evidence that the Epstein-Barr virus (EBV) is implicated in this disease [1-3]. As yet, no one has successfully transmitted EBV from patients with IM to cell cultures [2]. As a result, epidemiologic and clinical investigations have been limited mainly to serologic studies. This paper reports studies in which throat washings from IM patients converted an indicator lymphoid cell from negative to positive for EBV antigen.
Nineteen lines of human fibroblasts were inoculated with a filtrate prepared from the ST feline sarcoma. Seven lines showed morphological alteration and released focus-forming activity for feline cells, 2 lines showed morphological alteration but did not release focus-forming activity, and 11 lines showed no morphological alteration and released no focus-forming agent. Morphologically altered cells appeared enlarged, hyper-refractile, and intensely stained by hematoxylin. They neither assumed a crisscross pattern nor piled up to form a visible focus. Time-lapse cinegraph showed that the morphologically altered cells did not divide and were motile. The fluid from two human fibroblast cultures, inoculated 4 and 14 weeks previously with the ST sarcoma filtrate, induced fibrosarcoma in newborn kittens.
The relative capacity of several types of human cells and tissue to produce interferon was studied. Types of cells and tissue included were fibroblasts from embryos, foreskins, and biopsied skins; amnion cells; peripheral leukocytes; established lymphoid cell lines; established heteroploid cell lines; and chorioamniotic membrane. When Newcastle disease virus was used as the inducer, fibroblasts and amnion cells produced more interferon per 10(6) cells than leukocytes, lymphoid cells, and heteroploid cells. Only minor variations in interferon-producing capacity were observed among fibroblasts from 36 persons. Culture passage level, cell concentration, and inducer were factors that significantly affected interferon production.