Separation and Analysis of Peptides and ProteinsADVERTISEMENT RETURN TO ISSUEPREVReviewSeparation and Analysis of Peptides and ProteinsCynthia K. Larive, Susan M. Lunte, Min Zhong, Melissa D. Perkins, George S. Wilson, Giridharan Gokulrangan, Todd Williams, Farhana Afroz, Christian Schöneich, Tiffany S. Derrick, C. Russell Middaugh, and Susan Bogdanowich-KnippView Author Information Department of Chemistry, University of Kansas, Lawrence, Kansas 66045 Cite this: Anal. Chem. 1999, 71, 12, 389–423Publication Date (Web):May 19, 1999Publication History Published online19 May 1999Published inissue 1 June 1999https://pubs.acs.org/doi/10.1021/a1990013ohttps://doi.org/10.1021/a1990013oreview-articleACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views2532Altmetric-Citations74LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Chromatography,Genetics,Membranes,Peptides and proteins,Protein structure Get e-Alerts
Medium-throughput pKa screening of pharmaceuticals by pressure-assisted capillary electrophoresisA fast screening method for the determination of the dissociation constants (pKa) of acidic, basic, and multivalent compounds was developed by using pressure-assisted capillary electrophoresis (PACE). External air pressure was applied to shorten the analysis time. The separation efficiency decreases as air pressure increases. However, it was found that air pressure does not affect the measurement of electrophoretic mobility and pKa significantly when it is less than 2 psi. The method was evaluated in terms of accuracy, precision, and ruggedness by using a set of 48 compounds with literature pKa values ranging from 2 to 10. The difference between the measured pKa values and literature values is less than 0.2 units. The throughput is approximately 20 compounds per day with a 12-point measurement ranging from pH 2.5 to 11. It was demonstrated that this method is applicable for pKa screening of pharmaceuticals with diverse chemical structures.