Paul A. Wilks

(1967). Internal Reflection Spectroscopy. Applied Spectroscopy Reviews: Vol. 1, No. 1, pp. 99-130.

JCAMP-DX is a standard file form for exchange of infrared spectra and related chemical and physical information between spectrometer data systems of different manufacture, main-frame time-sharing systems, general purpose lab computers, and personal computers. It is compatible with all media: telephone, magnetic and optical disk, magnetic tape, and even the printed page (via optical reader). All data are stored as labeled fields of variable length using printable ASCII characters. A JCAMP-DX spectrum is a text file which can be viewed, corrected, and annotated with a text editor. The present focus is on infrared spectra, but JCAMP-DX can easily accommodate Raman, UV, NMR, mass, and other types of spectra, x-ray powder patterns, chromatograms, thermograms, and other plots which require the capability of representing contours as well as peak position and intensity. JCAMP-DX also provides for combining adequate information about the sample and method of observation with its spectrum.

Infrared spectra of aqueous antibiotic solutions were obtained using a new Liquid Analyzer accessory in a Fourier transform infrared (FT-IR) spectrometer. Based on a cylindrical internal reflectance element, the Liquid Analyzer accessory provides a sufficiently short (∼0.015 mm) effective pathlength for infrared spectroscopy of aqueous solutions from 3200 to 800 cm −1 , Spectra of aqueous solutions of penicillin VK, sodium oxacillin and sodium methicillin are shown. By measuring the absorbante of the beta-lactam carbonyl band, FT-IR spectroscopy with the accessory exhibits analytical sensitivity to less than 0.1% concentration by weight of antibiotic both in aqueous solution and in an actual fermentation broth.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConstruction and Performance of an Infrared Chromatographic Fraction Analyzer.P. A. Wilks and R. A. BrownCite this: Anal. Chem. 1964, 36, 10, 1896–1899Publication Date (Print):September 1, 1964Publication History Published online1 May 2002Published inissue 1 September 1964https://doi.org/10.1021/ac60216a007RIGHTS & PERMISSIONSArticle Views20Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (458 KB) Get e-Alerts Get e-Alerts

Internal reflection spectroscopy (also known as attenuated total reflection—ATR) has been applied primarily as a qualitative tool. As equipment for making internal reflection measurements improves and as the phenomenon is more clearly understood, its use for quantitative analysis is becoming more feasible. Katlafsky and Keller have demonstrated its accuracy for sampling strongly absorbing liquids and Medick has discussed its use in the quantitative analysis of adhesives and films. Chan and Hawkins have used internal reflection spectroscopy to obtain quantitative data on the degradation of plastic surfaces. Much unpublished work has been done in the measurement of coating thickness, in some cases quantitative studies have been made on multilayer systems. Wilks has described techniques for making quantitative measurements on mixed textile fabrics, while McCall et al. have discussed quantitative measurements of coatings and chemical treatments on cotton fibers.