Infrared Spectroscopy of Aqueous Antibiotic SolutionsInfrared spectra of aqueous antibiotic solutions were obtained using a new Liquid Analyzer accessory in a Fourier transform infrared (FT-IR) spectrometer. Based on a cylindrical internal reflectance element, the Liquid Analyzer accessory provides a sufficiently short (∼0.015 mm) effective pathlength for infrared spectroscopy of aqueous solutions from 3200 to 800 cm −1 , Spectra of aqueous solutions of penicillin VK, sodium oxacillin and sodium methicillin are shown. By measuring the absorbante of the beta-lactam carbonyl band, FT-IR spectroscopy with the accessory exhibits analytical sensitivity to less than 0.1% concentration by weight of antibiotic both in aqueous solution and in an actual fermentation broth.
A New Approach to Infrared Microspectroscopy: Adding FT-IR to a Light MicroscopeJournal Article A New Approach to Infrared Microspectroscopy: Adding FT-IR to a Light Microscope Get access John A Reffner, John A Reffner SensIR Technologies, Danbury CT 06810 Search for other works by this author on: Oxford Academic Google Scholar Donald K Wilks, Donald K Wilks SensIR Technologies, Danbury CT 06810 Search for other works by this author on: Oxford Academic Google Scholar Kenneth C Schreiber, Kenneth C Schreiber SensIR Technologies, Danbury CT 06810 Search for other works by this author on: Oxford Academic Google Scholar Robert V Burch Robert V Burch SensIR Technologies, Danbury CT 06810 Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 8, Issue S02, 1 August 2002, Pages 1526–1527, https://doi.org/10.1017/S1431927602104259 Published: 01 August 2002