Jiamin Lou

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide, leading to a large global cancer burden. Hepatocyte growth factor (HGF) and its high-affinity receptor, mesenchymal epithelial transition factor (c-Met), are closely related to the onset, progression, and metastasis of multiple tumors. The HGF/c-Met axis is involved in cell proliferation, movement, differentiation, invasion, angiogenesis, and apoptosis by activating multiple downstream signaling pathways. In this review, we focus on the function of the HGF/c-Met axis in HCC. The HGF/c-Met axis promotes the onset, proliferation, invasion, and metastasis of HCC. Moreover, it can serve as a biomarker for diagnosis and prognosis, as well as a therapeutic target for HCC. In addition, it is closely related to drug resistance during HCC treatment.

nanoparticles with ginsenoside Rg3 (NpRg3), which achieves an excellent coupling effect. In the dimethylnitrosamine-induced HCC model, NpRg3 application significantly prolongs the survival of HCC mice. Further research indicates that NpRg3 application significantly inhibits HCC development and eliminates HCC metastasis to the lung. Notably, NpRg3 application delays HCC-induced ileocecal morphology and gut microbial alterations more than 12 weeks during HCC progression. NpRg3 administration elevates the abundance of Bacteroidetes and Verrucomicrobia, but decreases Firmicutes. Twenty-nine predicted microbial gene functions are enriched, while seven gene functions are reduced after NpRg3 administration. Moreover, the metabolomics profile presents a significant progression during HCC development, but NpRg3 administration corrects tumor-dominant metabolomics. NpRg3 administration decreases 3-indolepropionic acid and urea, but elevates free fatty acids. Importantly, NpRg3 application remodels the unbalanced correlation networks between gut microbiota and metabolism during HCC therapy. In conclusion, nanoparticle conjugation of ginsenoside Rg3 inhibits HCC development and metastasis via the remodeling of unbalanced gut microbiota and metabolism in vivo, providing an antitumor therapy strategy.

Objective: The intestinal microbiome is associated with various autoimmune diseases. Regional difference is the main influencing factor of intestinal microbial difference. This study aimed to identify the differences in fecal microbiome between autoimmune hepatitis (AIH) patients and healthy controls (HCs) in Central China, and to validate the efficacy of fecal microbiome as a diagnostic tool for AIH. Design: We collected 115 fecal samples from AIH patients (N = 37) and HCs (N = 78) in Central China and performed gene sequencing. Fecal microbiomes were characterized and microbial markers for AIH were identified. Results: Fecal microbial diversity showed a downward trend in AIH compared with HCs. Fecal microbial communities significantly differed between both groups. At the phylum level, Verrucomicrobia abundance was significantly increased, while Lentisphaerae and Synergistetes were significantly decreased in the AIH patients versus the HCs. Compared to the HCs, fifteen genera, including Veillonella, Faecalibacterium and Akkermansia, were enriched, while nineteen genera, such as Pseudobutyrivibrio, Lachnospira and Ruminococcaceae, were decreased in the AIH patients. Ten genera, including Veillonella, Faecalibacterium and Akkermansia, predominated in the AIH patients. Five microbial biomarkers were deemed optimal diagnostic tools for AIH. The probability of disease was significantly increased in AIH group versus HCs, achieving 83.25% value of area under the curve. Conclusion: We present the characteristics of AIH patients in Central China for the first time. Five microbial biomarkers, including Lachnospiraceae, Veillonella, Bacteroides, Roseburia and Ruminococcaceae, achieved a high potential distinguishing AIH patients from HCs.

Food waste is a major problem faced by human beings. Acidogenic fermentation is an effective and feasible technology for resource recovery from food waste. The mixture of volatile fatty acids (VFAs) hinders the utilization of fermentation products. In this study, we constructed fermentation reactors for food waste treatment. The operation period was separated to three stages: Stage 1 (from day 1–102), Stage 2 (from day 103–208), and Stage 3 (from day 209–304). CO 2 was sparged to the reactors to promote the acetate enrichment at Stage 3. Bioinformatics analysis were performed to analyze the microbial community, genes, and pathways. Results showed that the highest average concentration of acetate was 6044 mg-COD/L (R1) and 5000 mg-COD/L (R2) at Stage 3, which was corresponded to the stage with highest acetate ratio (63% and 66% in R1 and R2). But the highest total VFAs concentration was 39424 mg-COD/L at Stage 2. Aeriscardovia belonging to Actinobacteria had an average relative abundance of 85.7% after CO 2 sparging. Compared with Stage 1 and Stage 2, the number of down-regulated genes and pathways at Stage 3 were much higher than the number of up-regulated genes and pathways. The significant down-regulated genes were wcaB and ttrC, and the significant down-regulated pathways were pyruvate fermentation to acetone and acetyl-CoA fermentation to butanoate II pathway. This study demonstrated that CO 2 can promote the acetate enrichment during food waste fermentation. The main mechanism was enriching acetate fermentation microorganisms and inhibiting the interfere genes and pathways. • CO 2 sparging contributed to the selective enrichment of acetate. • pH below 4 was beneficial for total VFAs accumulation. • Microbial community vary greatly after CO 2 sparging. • The main regulation mechanism of acetate enrichment was down-regulated genes and pathways.