The influence of carbon dioxide on fermentation products, microbial community, and functional gene in food waste fermentation with uncontrol pH

Abstract

Food waste is a major problem faced by human beings. Acidogenic fermentation is an effective and feasible technology for resource recovery from food waste. The mixture of volatile fatty acids (VFAs) hinders the utilization of fermentation products. In this study, we constructed fermentation reactors for food waste treatment. The operation period was separated to three stages: Stage 1 (from day 1–102), Stage 2 (from day 103–208), and Stage 3 (from day 209–304). CO 2 was sparged to the reactors to promote the acetate enrichment at Stage 3. Bioinformatics analysis were performed to analyze the microbial community, genes, and pathways. Results showed that the highest average concentration of acetate was 6044 mg-COD/L (R1) and 5000 mg-COD/L (R2) at Stage 3, which was corresponded to the stage with highest acetate ratio (63% and 66% in R1 and R2). But the highest total VFAs concentration was 39424 mg-COD/L at Stage 2. Aeriscardovia belonging to Actinobacteria had an average relative abundance of 85.7% after CO 2 sparging. Compared with Stage 1 and Stage 2, the number of down-regulated genes and pathways at Stage 3 were much higher than the number of up-regulated genes and pathways. The significant down-regulated genes were wcaB and ttrC, and the significant down-regulated pathways were pyruvate fermentation to acetone and acetyl-CoA fermentation to butanoate II pathway. This study demonstrated that CO 2 can promote the acetate enrichment during food waste fermentation. The main mechanism was enriching acetate fermentation microorganisms and inhibiting the interfere genes and pathways. • CO 2 sparging contributed to the selective enrichment of acetate. • pH below 4 was beneficial for total VFAs accumulation. • Microbial community vary greatly after CO 2 sparging. • The main regulation mechanism of acetate enrichment was down-regulated genes and pathways.