Ribosomal RNA Depletion for Efficient Use of RNA‐Seq CapacityDominic O’Neil, Heike Glowatz, Martin Schlumpberger|Current Protocols in Molecular Biology|2013 Ribosomal RNA (rRNA) is the most highly abundant component of RNA, comprising the majority (>80% to 90%) of the molecules present in a total RNA sample. Depletion of this rRNA fraction is desirable prior to performing an RNA-seq reaction, so that sequencing capacity can be focused on more informative parts of the transcriptome. This unit describes an rRNA depletion method based on selective hybridization of oligonucleotides to rRNA, recognition with a hybrid-specific antibody, and removal of the antibody-hybrid complex on magnetic beads.
Dynamic model development for a continuous culture of <i>Saccharomyces cerevisiae</i>Dominic O’Neil, G. Lyberatos|Biotechnology and Bioengineering|1990 The problem of dynamically modeling a chemostat is addressed. Using the results of continuous culture experiments for the growth of a strain of Saccharomyces cerevisiae on a glucose-limited medium, a general approach to developing dynamic models is discussed. The approach to develop and verify the model involves three different types of experiments: steady-state, dynamic step response, and feedback identification.