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Bruce A. Buchholz

Université Claude Bernard Lyon 1

ORCID: 0000-0002-9531-551X

Publishes on Mass Spectrometry Techniques and Applications, Radioactive contamination and transfer, Advanced Combustion Engine Technologies. 232 papers and 13.8k citations.

232Publications
13.8kTotal Citations

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Top publicationsby citations

Evidence for Cardiomyocyte Renewal in Humans
Cited by 3.1kOpen Access

It has been difficult to establish whether we are limited to the heart muscle cells we are born with or if cardiomyocytes are generated also later in life. We have taken advantage of the integration of carbon-14, generated by nuclear bomb tests during the Cold War, into DNA to establish the age of cardiomyocytes in humans. We report that cardiomyocytes renew, with a gradual decrease from 1% turning over annually at the age of 25 to 0.45% at the age of 75. Fewer than 50% of cardiomyocytes are exchanged during a normal life span. The capacity to generate cardiomyocytes in the adult human heart suggests that it may be rational to work toward the development of therapeutic strategies aimed at stimulating this process in cardiac pathologies.

Neocortical neurogenesis in humans is restricted to development
Ratan D. Bhardwaj, Maurice A. Curtis, Kirsty L. Spalding et al.|Proceedings of the National Academy of Sciences|2006
Cited by 445Open Access

Stem cells generate neurons in discrete regions in the postnatal mammalian brain. However, the extent of neurogenesis in the adult human brain has been difficult to establish. We have taken advantage of the integration of (14)C, generated by nuclear bomb tests during the Cold War, in DNA to establish the age of neurons in the major areas of the human cerebral neocortex. Together with the analysis of the neocortex from patients who received BrdU, which integrates in the DNA of dividing cells, our results demonstrate that, whereas nonneuronal cells turn over, neurons in the human cerebral neocortex are not generated in adulthood at detectable levels but are generated perinatally.