Bian Rongwen

Objective To observe the influence of acupuncture intervention on the fat content and biochemical metabolism in metabolic syndrome(MS).Methods Twenty-two patients with metabolic syndrome were treated with acupuncture.Twenty-four patients were treated with Metformin as a control.Results The clinical symptoms and signs improved obviously in the two groups of MS patients, but the effect was better in the acupuncture group than in the medication group(P0.05).The changes in obesity indices and biochemical indices showed that body mass index was decreased more in the acupuncture group than in the medication group(P0.05), but fasting blood sugar was not regulated so well in the former as in the latter(P0.05).Conclusion Acupuncture intervention produces an active effect on the fat content and biochemical metabolism in metabolic syndrome but does not regulate fasting blood sugar so well as medication.

Objective:Investigate the effects of winged euonymus twig on glucose uptake of human adipocytes.Methods:The human adipocytes were separated and treated with 100nmol·L-1 amylin and 1.5U/L insulin then put in winged euonymus twig and the glucose uptake was determined.Result:It was found that amylin can inhibited glucose uptake,while winged euonymus twig can ameliorate the amylin inhibition.But no statistical significance.Conclusion:Amylin can inhibit the glucose transport in human adipocytes,while winged euonymus twig can not obviously improve the inhibition.

The microsatellite polymorphism genetic markers of glucokinase (GCK,MODY2) and hepatocyte nuclear factor1#alpha# (HNF-1#alpha#,MODY3) genes were selcted to perform the classical linkage analysis in the NIDDM/MODY family 50001.Direct sequencing was employed to confirm the variation of nucleotide(s) in the promoter and all the exons of HNF-1#alpha# gene.No linkage was found in GCK genetic marker with diabetes in family 50001.The maximal LOD(logarithm of odds) score was 2.375 930(0=0.000 000) for the HNF-1#alpha# genetic marker with diabetes at the mode of autosomal dominant in family 50001.By direct sequencing,a heterozygous polymorphism,Ser487 Asn (AGC/AAC),was indicated in exon 7,and it could be found in the diabetic and normal subjects as well.