High-definition spatial transcriptomics for in situ tissue profiling
Sanja Vicković(Broad Institute), Gökçen Eraslan(Broad Institute), Fredrik Salmén(Science for Life Laboratory), Johanna Klughammer(Broad Institute), Linnea Stenbeck(Science for Life Laboratory), Denis Schapiro(Broad Institute), Tarmo Äijö(Flatiron Health (United States)), Richard Bonneau(Brigham and Women's Hospital), Ludvig Bergenstråhle(Science for Life Laboratory), José Fernández Navarro(Science for Life Laboratory), Joshua Gould(Broad Institute), Gabriel K. Griffin(Broad Institute), Åke Borg(Lund University), Mostafa Ronaghi(Illumina (United States)), Jonas Frisén(Karolinska Institutet), Joakim Lundeberg(KTH Royal Institute of Technology), Aviv Regev(Broad Institute), Patrik L. Ståhl(Science for Life Laboratory)
Cited by 1,210Open Access
Abstract
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues. A dense, spatially barcoded bead array captures RNA from histological tissue sections for spatially resolved gene expression analysis.