Single Primer Enrichment Technology (SPET) for High-Throughput Genotyping in Tomato and Eggplant Germplasm
Lorenzo Barchi(Forest and Range Management Research Institute), Alberto Acquadro(Forest and Range Management Research Institute), David Alonso(Universitat Politècnica de València), Giuseppe Aprea(National Agency for New Technologies, Energy and Sustainable Economic Development), Laura Bassolino, Olivia Demurtas(National Agency for New Technologies, Energy and Sustainable Economic Development), Paola Ferrante(National Agency for New Technologies, Energy and Sustainable Economic Development), Pietro Gramazio(Universitat Politècnica de València), Paola Mini(National Agency for New Technologies, Energy and Sustainable Economic Development), Ezio Portis(Forest and Range Management Research Institute), Davide Scaglione(Istituto di Genomica Applicata), Laura Toppino, Santiago Vilanova(Universitat Politècnica de València), María José Díez(Universitat Politècnica de València), Giuseppe Leonardo Rotino, Sergio Lanteri(University of Turin), Jaime Prohens(Universitat Politècnica de València), Giovanni Giuliano(National Agency for New Technologies, Energy and Sustainable Economic Development)
Cited by 130Open Access
Abstract
for eggplant). Maximum Likelihood phylogenetic trees and PCA outputs obtained from the whole dataset highlighted genetic relationships among accessions and species which were congruent with what was previously reported in literature. Better discrimination among domesticated accessions was achieved by using the target SNPs, while better discrimination among wild species was achieved using the whole SNP dataset. Our results reveal that SPET genotyping is a robust, high-throughput technology for genetic fingerprinting, with a high degree of cross-transferability between crops and their cultivated and wild relatives, and allows identification of duplicates and mislabeled accessions in genebanks.