Guidelines for morpholino use in zebrafish
Didier Y. R. Stainier(Max Planck Institute for Heart and Lung Research), Erez Raz(University of Münster), Nathan D. Lawson(University of Massachusetts Chan Medical School), Stephen C. Ekker(Mayo Clinic), Rebecca D. Burdine(Princeton University), Judith S Eisen(University of Oregon), Philip W. Ingham(Nanyang Technological University), Stefan Schulte‐Merker, Deborah Yelon(University of California San Diego), Brant M. Weinstein(Eunice Kennedy Shriver National Institute of Child Health and Human Development), Mary C. Mullins(University of Pennsylvania), Stephen W. Wilson(University College London), Lalita Ramakrishnan(MRC Laboratory of Molecular Biology), Sharon L. Amacher(The Ohio State University), Stephan C. F. Neuhauss(University of Zurich), Anming Meng(Tsinghua University), Naoki Mochizuki(National Cerebral and Cardiovascular Center), Pertti Panula(University of Helsinki), Cecilia B. Moens(Fred Hutch Cancer Center)
Cited by 337Open Access
Abstract
The zebrafish (Danio rerio) has emerged as a powerful model to study vertebrate development and disease. Its short generation time makes it amenable to genetic manipulation and analysis, and its small size and high fecundity make it especially well suited for large-scale forward genetic and chemical screens. Fast-developing zebrafish embryos are transparent, facilitating live imaging of a variety of developmental processes in wild-type and mutant animals. ...\nThis brief document provides an updated set of guidelines regarding morpholino use in zebrafish that we anticipate will be of value for experimentalists as well as journal and grant reviewers, and decision makers.