Multilineage Cells from Adipose Tissue as Gene Delivery Vehicles

Kouki Morizono(Black AIDS Institute), Daniel A. De Ugarte(University of California, Los Angeles), Min Zhu(University of California, Los Angeles), Pat Zuk(University of California, Los Angeles), Amir Elbarbary(University of California, Los Angeles), Peter Ashjian(University of California, Los Angeles), Prosper Benhaim(University of California, Los Angeles), Irvin S. Y. Chen(Black AIDS Institute), Marc H. Hedrick(University of California, Los Angeles)
Human Gene Therapy
January 1, 2003
10.1089/10430340360464714
Cited by 148

Abstract

We have characterized a population of mesenchymal progenitor cells from adipose tissue, termed processed lipoaspirate (PLA) cells, which have multilineage potential similar to bone marrow-derived mesenchymal stem cells and are also easily expanded in culture. The primary benefit of using adipose tissue as a source of multilineage progenitor cells is its relative abundance and ease of procurement. We examined the infection of PLA cells with adenoviral, oncoretroviral, and lentiviral vectors. We demonstrate that PLA cells can be transduced with lentiviral vectors at high efficiency. PLA cells maintain transgene expression after differentiation into adipogenic and osteogenic lineages after lentiviral transduction. Therefore, PLA cells and lentiviral vectors may be an efficient combination for use as a therapeutic gene delivery vehicle.

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