Two Closely Related Subclass II SnRK2 Protein Kinases Cooperatively Regulate Drought-Inducible Gene Expression

Masahide Mizoguchi, Taishi Umezawa, Kazuo Nakashima(Japan International Research Center for Agricultural Sciences), Satoshi Kidokoro(The University of Tokyo), Hironori Takasaki(The University of Tokyo), Yasunari Fujita(Japan International Research Center for Agricultural Sciences), Kazuko Yamaguchi‐Shinozaki(The University of Tokyo), Kazuo Shinozaki
Plant and Cell Physiology
April 7, 2010
10.1093/pcp/pcq041
Cited by 164

Abstract

The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling in Arabidopsis; however, the roles of other subclasses remain elusive. Here, we established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) and their targets, and quantitative reverse transcription- PCR confirmed that those genes were down-regulated significantly in srk2cf. This study indicates that subclass II SnRK2s also play important roles in drought stress signaling in Arabidopsis.

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