A Map of the Interactome Network of the Metazoan <i>C. elegans</i>

Siming Li(Harvard University), Christopher M. Armstrong(Harvard University), Nicolas Bertin(Harvard University), Hui Ming Ge(Harvard University), Stuart Milstein(Harvard University), Mike Boxem(Harvard University), Pierre‐Olivier Vidalain(Harvard University), Jing-Dong Han(Harvard University), Alban Chesneau(Harvard University), Tong Hao(Harvard University), Debra S. Goldberg(Harvard University), Ning Li(Harvard University), Monica Martinez(Harvard University), Jean‐François Rual(Harvard University), Philippe Lamesch(Harvard University), Lai Xu(Harvard University), Muneesh Tewari(Harvard University), S. L. Wong(Harvard University), Lan V. Zhang(Harvard University), Gabriel F. Berriz(Harvard University), Laurent Jacotot(Harvard University), Philippe Vaglio(Harvard University), Jérôme Reboul(Harvard University), Tomoko Hirozane-Kishikawa(Harvard University), Qianru Li(Harvard University), Harrison W. Gabel(Harvard University), Ahmed Elewa(Harvard University), Bridget Baumgartner(Harvard University), Debra J. Rose(Harvard University), Haiyuan Yu(Harvard University), Stephanie Bosak(Harvard University), Reynaldo Sequerra(Harvard University), Andrew Fraser(Harvard University), Susan E. Mango(Harvard University), William M. Saxton(Harvard University), Susan Strome(Harvard University), Sander van den Heuvel(Harvard University), Fabio Piano(Harvard University), Jean Vandenhaute(Harvard University), Claude Sardet(Harvard University), Mark Gerstein(Harvard University), Lynn Doucette‐Stamm(Harvard University), Kristin C. Gunsalus(Harvard University), J. Wade Harper(Harvard University), M. E. Cusick(Harvard University), Frederick P. Roth(Harvard University), David E. Hill(Harvard University), Marc Vidal(Harvard University)
Science
January 5, 2004
10.1126/science.1091403
Cited by 1,652Open Access
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Abstract

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains approximately 5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

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