Detecting Protein Function and Protein-Protein Interactions from Genome Sequences
Edward M. Marcotte(University of California, Los Angeles), Matteo Pellegrini(University of California, Los Angeles), Ho Leung Ng(University of California, Los Angeles), Danny W. Rice(University of California, Los Angeles), Todd O. Yeates(University of California, Los Angeles), David Eisenberg(University of California, Los Angeles)
Cited by 1,678
Abstract
A computational method is proposed for inferring protein interactions from genome sequences on the basis of the observation that some pairs of interacting proteins have homologs in another organism fused into a single protein chain. Searching sequences from many genomes revealed 6809 such putative protein-protein interactions in Escherichia coli and 45,502 in yeast. Many members of these pairs were confirmed as functionally related; computational filtering further enriches for interactions. Some proteins have links to several other proteins; these coupled links appear to represent functional interactions such as complexes or pathways. Experimentally confirmed interacting pairs are documented in a Database of Interacting Proteins.