Laser Capture Microdissection
Michael R. Emmert‐Buck(National Cancer Institute), Robert F. Bonner(Foundation for the National Institutes of Health), Paul D. Smith(Foundation for the National Institutes of Health), Rodrigo Chuaqui(National Cancer Institute), Zhengping Zhuang(National Cancer Institute), Seth R. Goldstein(Foundation for the National Institutes of Health), R. Weiss(National Cancer Institute), Lance A. Liotta(National Cancer Institute)
Cited by 2,562
Abstract
Laser capture microdissection (LCM) under direct microscopic visualization permits rapid one-step procurement of selected human cell populations from a section of complex, heterogeneous tissue. In this technique, a transparent thermoplastic film (ethylene vinyl acetate polymer) is applied to the surface of the tissue section on a standard glass histopathology slide; a carbon dioxide laser pulse then specifically activates the film above the cells of interest. Strong focal adhesion allows selective procurement of the targeted cells. Multiple examples of LCM transfer and tissue analysis, including polymerase chain reaction amplification of DNA and RNA, and enzyme recovery from transferred tissue are demonstrated.