Genomic analysis identifies association of <i>Fusobacterium</i> with colorectal carcinoma

Aleksandar D. Kostic(Broad Institute), Dirk Gevers(Broad Institute), Chandra Sekhar Pedamallu(Broad Institute), Monia Michaud(Harvard University), Fujiko Duke(Broad Institute), Ashlee M. Earl(Broad Institute), Akinyemi I. Ojesina(Broad Institute), Joonil Jung(Broad Institute), Adam J. Bass(Broad Institute), Josep Tabernero(Universitat Autònoma de Barcelona), José Baselga(Universitat Autònoma de Barcelona), Chen Liu(University of Florida), Ramesh A. Shivdasani(Dana-Farber Cancer Institute), Shuji Ogino(Brigham and Women's Hospital), Bruce W. Birren(Broad Institute), Curtis Huttenhower(Broad Institute), Wendy S. Garrett(Broad Institute), Matthew Meyerson(Broad Institute)
Genome Research
October 18, 2011
10.1101/gr.126573.111
Cited by 1,957Open Access
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Abstract

The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.

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