MRI Detection of Thrombin with Aptamer Functionalized Superparamagnetic Iron Oxide Nanoparticles

Mehmet V. Yigit(University of Illinois Urbana-Champaign), Debapriya Mazumdar(University of Illinois Urbana-Champaign), Yi Lu(University of Illinois Urbana-Champaign)
Bioconjugate Chemistry
January 4, 2008
Cited by 175

Abstract

Design of smart MRI contrast agent based on superparamagnetic iron oxide nanoparticles and aptamers has been described for the detection of human alpha-thrombin protein. The contrast agent is based on the assembly of the aptamer functionalized nanoparticles in the presence of thrombin. A detectable change in MRI signal is observed with 25 nM thrombin in human serum. Changes were neither observed with control analytes, streptavidin, or bovine serum albumin, nor with inactive aptamer functionalized nanoparticles.


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