Kidney produces a novel acylated peptide, ghrelin

Kiyoshi Mori(Kyoto University), Akihiro Yoshimoto(Kyoto University), Kazuhiko Takaya(Kyoto University), Kiminori Hosoda(Kyoto University), Hiroyuki Ariyasu(Kyoto University), Kensei Yahata(Kyoto University), Masashi Mukoyama(Kyoto University), Akira Sugawara(Kyoto University), Hiroshi Hosoda(National Cerebral and Cardiovascular Center), Masayasu Kojima(National Cerebral and Cardiovascular Center), Kenji Kangawa(National Cerebral and Cardiovascular Center), Kazuwa Nakao(Kyoto University)
FEBS Letters
December 12, 2000
10.1016/s0014-5793(00)02308-5
Cited by 333

Abstract

Ghrelin is a novel growth hormone-releasing peptide with a unique acylated structure. Here we reveal that prepro-ghrelin gene is expressed in the mouse kidney and glomerulus. We also show by reverse-phase high performance liquid chromatography coupled with radioimmunoassay that the mouse kidney does produce ghrelin. The ghrelin immunoreactivity in the mouse kidney is 6.79+/-0.48 fmol/mg (n=5), which is much more abundant than that in the mouse plasma of 0.339+/-0.029 fmol/microl (n=6). Furthermore, prepro-ghrelin gene is expressed in cultured rat mesangial cells, fibroblast-like NRK-49F cells and mouse podocytes, but not in rat epithelial cell-like NRK-52E cells. Ghrelin receptor gene is also expressed in the rat kidney. These findings demonstrate that the kidney, glomerulus and renal cells express prepro-ghrelin gene and ghrelin is produced locally in the kidney, and suggest the endocrine and/or paracrine roles of ghrelin in the kidney.

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