Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun Collections
Frederick R. Blattner(University of Wisconsin–Madison), Ann E. Blechl(University of Wisconsin–Madison), Katherine Denniston-Thompson(University of Wisconsin–Madison), Harvey E. Faber(University of Wisconsin–Madison), Julia E. Richards(University of Wisconsin–Madison), Jerry L. Slightom(University of Wisconsin–Madison), Phillip W. Tucker(University of Wisconsin–Madison), Oliver Smithies(University of Wisconsin–Madison)
Cited by 349
Abstract
Shotgun collections of Charon 3A bacteriophages containing Eco RI fragments of human and mouse DNA were constructed with the use of in vitro packaging. Plaques were screened by hybridization, and globin-specific clones were isolated from both human (Charon 3AHs51.1) and mouse (Charon 3AMm30.5). The fragments cloned were detected in unfractionated genomic DNA by the Southern method of hybridization.