Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA Cloning
Frederick R. Blattner(University of Wisconsin–Madison), Bill G. Williams(University of Wisconsin–Madison), Ann E. Blechl(University of Wisconsin–Madison), Katherine Denniston-Thompson(University of Wisconsin–Madison), Harvey E. Faber(University of Wisconsin–Madison), Lesley-Anne Furlong(University of Wisconsin–Madison), David J. Grunwald(University of Wisconsin–Madison), Delight O. Kiefer(University of Wisconsin–Madison), David D. Moore(University of Wisconsin–Madison), James W. Schumm(University of Wisconsin–Madison), Edward L. Sheldon(University of Wisconsin–Madison), Oliver Smithies(University of Wisconsin–Madison)
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Abstract
The Charon lambda bacteriophages have been developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector phages, 3A, 4A, and 16A, have been certified for use as EK2 vector-host systems, when propagated in bulk in a special bacterial host, DP50SupF. We present here some of the data on which the safety of these systems was evaluated. DNA fragments ranging in size from 0 to 2.2 X 10(4) base pairs can be cloned in these EK2 Charon phages.
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