A Neutralizing Antibody Selected from Plasma Cells That Binds to Group 1 and Group 2 Influenza A Hemagglutinins

Davide Corti, Jarrod Voss(National Institute for Medical Research), S.J. Gamblin(National Institute for Medical Research), Giosiana Codoni, Annalisa Macagno, David Jarrossay, S.G. Vachieri(National Institute for Medical Research), Debora Pinna, Andrea Minola, Fabrizia Vanzetta, Chiara Silacci, Blanca Fernandez‐Rodriguez, Gloria Agatic, Siro Bianchi, Isabella Giacchetto-Sasselli, Lesley J. Calder(National Institute for Medical Research), Federica Sallusto, Patrick Collins(National Institute for Medical Research), L.F. Haire(National Institute for Medical Research), Nigel Temperton(Medway School of Pharmacy), Johannes P. M. Langedijk(Pepscan (Netherlands)), J.J. Skehel(National Institute for Medical Research), Antonio Lanzavecchia(Board of the Swiss Federal Institutes of Technology)
Science
July 28, 2011
10.1126/science.1205669
Cited by 1,229

Abstract

The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.

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