Experimental study for the toxicological effects of SVATE-3 on rat kidney. Observation by light and electron microscope and by enzyme histocytochemistry.

Abstract

We have done an acute toxicological experiment on the rat kidney by administration of SVATE-3 in clinical dose (high and low dose, respectively) for 3 weeks. By using morphological methods, light and electron microscopy, enzyme morphological methods, light and electron microscopy, enzyme histocytochemistry in light and electron microscope and biochemistry, we have observed the histological structure and ultrastucture of rat kidney as well as the activity and distribution of the index enzymes for the kidney cell membrane such as Mg-adenosine triphosphatase, 5′-nucleotidase, alkaline phosphatase and glucose-6-phosphatase for kidney cell organelle: endoplasmic reticulum. Futhermore, the biochemical quantitation has been given. The results show that there is no toxic damage to the histological structure and enzyme activity and distribution of rat kidney by administration of SVATE-3 in the low dose. But in high dose, the filter membrane of the kidney was damaged and the activity of Mg-ATPase were decreased. This suggests that we should pay more attention to SVATE-3′s toxicological threshold value when using it and get safe treatment.