Leaderboards/Protein purification and stability

Protein purification and stability

Last updated July 15, 2026

Rising Stars

Fresh ink, massive impact

Researchers

1
Martin Pačesa
SIB Swiss Institute of Bioinformatics
2
Mahshid Askarizadeh
Islamic Azad University, Marvdasht
3
Emily K. Makowski
University of Michigan
4
Hogyun Seo
Kyungpook National University
5
Timon Damelang
The University of Melbourne
6
Jeffrey A. Ruffolo
Joint BioEnergy Institute
7
Andreas V. Madsen
Technical University of Denmark
8
Leif-Thore Deck
ETH Zurich
9
Honorine Lardeux
University of Geneva
10
Sofie Meulewaeter
Ghent University

Papers

1
One-shot design of functional protein binders with BindCraft
Nature|2025
158 cit.
2
Publication in Frontiers in Immunology (2024) [112 citations, W4390665680]
Frontiers in Immunology|2024
112 cit.
3
Designing proteins with language models
Nature Biotechnology|2024
97 cit.
4
Design of highly functional genome editors by modelling CRISPR–Cas sequences
Nature|2025
72 cit.
5
Design and engineering of bispecific antibodies: insights and practical considerations
Frontiers in Bioengineering and Biotechnology|2024
62 cit.
6
Recent advances and current challenges in hydrophilic interaction chromatography for the analysis of therapeutic oligonucleotides
TrAC Trends in Analytical Chemistry|2024
60 cit.
7
Unravelling the Link between Oligonucleotide Structure and Diastereomer Separation in Hydrophilic Interaction Chromatography
Analytical Chemistry|2024
43 cit.
8
Publication in Journal of Controlled Release (2024) [41 citations, W4396633335]
Journal of Controlled Release|2024
41 cit.
9
Reduction of monoclonal antibody viscosity using interpretable machine learning
mAbs|2024
34 cit.
10
Binary and ternary approach of solubility of Rivaroxaban for preparation of developed nano drug using supercritical fluid
Arabian Journal of Chemistry|2024
26 cit.

Emerging Leaders

Drafting the new consensus

Researchers

1
Elena Domínguez‐Vega
Leiden University Medical Center
2
Seth A. Lieblich
California Institute of Technology
3
Shunhai Wang
Regeneron (United States)
4
Douglas D. Richardson
United States Military Academy
5
Andrea Allmendinger
University of Freiburg
6
Ping Hu
Janssen (Belgium)
7
Stefano Menegatti
North Carolina State University
8
Laurence Fayadat‐Dilman
Merck & Co., Inc., Rahway, NJ, USA (United States)
9
Marina Kirkitadze
Sanofi (France)
10
Pietro Sormanni
University of Cambridge

Papers

1
Assessing antibody and nanobody nativeness for hit selection and humanization with AbNatiV
Nature Machine Intelligence|2024
74 cit.
2
Automated optimisation of solubility and conformational stability of antibodies and proteins
Nature Communications|2023
61 cit.
3
Fragment-based computational design of antibodies targeting structured epitopes
Science Advances|2022
52 cit.
4
A machine learning strategy for the identification of key <i>in silico</i> descriptors and prediction models for IgG monoclonal antibody developability properties
mAbs|2023
41 cit.
5
Approaches to expand the conventional toolbox for discovery and selection of antibodies with drug-like physicochemical properties
mAbs|2023
39 cit.
6
The downstream bioprocess toolbox for therapeutic viral vectors
Journal of Chromatography A|2023
34 cit.
7
Interrogating heterogeneity of cysteine-engineered antibody-drug conjugates and antibody-oligonucleotide conjugates by capillary zone electrophoresis-mass spectrometry
mAbs|2023
27 cit.
8
Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates
Biotechnology and Bioengineering|2023
27 cit.
9
Formulation screening of lyophilized mRNA-lipid nanoparticles
International Journal of Pharmaceutics|2025
27 cit.
10
Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via “flow‐through affinity chromatography” using peptide‐based adsorbents
Biotechnology and Bioengineering|2022
26 cit.

Current Senior Leaders

Wrote the book, still writing it

Researchers

1
Massimo Morbidelli
Aristotle University of Thessaloniki
2
John F. Carpenter
Atacama Large Millimeter Submillimeter Array
3
Theodore W. Randolph
University of Colorado Boulder
4
Andreas Plückthun
University of Zurich
5
Fred E. Regnier
University of Liège
6
Alois Jungbauer
BOKU University
7
Adi̇l Deni̇zli̇
Hacettepe University
8
Yasushi Ishihama
Kyoto University
9
Matthias Mann
Max Planck Institute of Biochemistry
10
Yan Sun
Taiwan Semiconductor Manufacturing Company (China)

Papers

1
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification
Nature Biotechnology|2008
15.7k cit.
2
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
Analytical Chemistry|1996
9.1k cit.
3
Universal sample preparation method for proteome analysis
Nature Methods|2009
8.7k cit.
4
Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment
Journal of Proteome Research|2011
5.9k cit.
5
Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics
Molecular & Cellular Proteomics|2002
5.6k cit.
6
Accurate Proteome-wide Label-free Quantification by Delayed Normalization and Maximal Peptide Ratio Extraction, Termed MaxLFQ
Molecular & Cellular Proteomics|2014
5.5k cit.
7
In-gel digestion for mass spectrometric characterization of proteins and proteomes
Nature Protocols|2006
5.1k cit.
8
Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips
Nature Protocols|2007
4.9k cit.
9
Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions
Science|2009
4k cit.
10
Global, In Vivo, and Site-Specific Phosphorylation Dynamics in Signaling Networks
Cell|2006
3.6k cit.

Reagents for Protein purification and stability

Citation-validated products used in this field

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