Leaderboards/Advanced Fluorescence Microscopy Techniques

Advanced Fluorescence Microscopy Techniques

Last updated July 15, 2026

Rising Stars

Fresh ink, massive impact

Researchers

1
Alissa Greenwald
University of Toronto
2
Lucas von Chamier
Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung
3
Eduard M. Unterauer
Center for NanoScience
4
Xinyang Li
Zhejiang University
5
Catxere A. Casacio
The University of Queensland
6
Jinrun Dong
Inner Mongolia University
7
Fabio Henrique dos Santos Rodrigues
Universidade Estadual de Campinas (UNICAMP)
8
Jan Otto Wolff
Max Planck Institute for Medical Research
9
Brendan Miller
Johns Hopkins University
10
Janne K. Lappalainen
Howard Hughes Medical Institute

Papers

1
Integrative spatial analysis reveals a multi-layered organization of glioblastoma
Cell|2024
303 cit.
2
Publication in Cell (2024) [143 citations, W4393251345]
Cell|2024
143 cit.
3
Humanin variant <scp>P3S</scp> is associated with longevity in <i>APOE4</i> carriers and resists <i>APOE4</i>‐induced brain pathology
Aging Cell|2024
16 cit.
4
ShortStop: a machine learning framework for microprotein discovery
BMC Methods|2025
5 cit.
5
Publication in Nature Communications (2025) [5 citations, W4414764973]
Nature Communications|2025
5 cit.
6
Publication in STAR Protocols (2025) [1 citation, W4408164866]
STAR Protocols|2025
1 cit.

Emerging Leaders

Drafting the new consensus

Researchers

1
Ali Ertürk
Helmholtz Zentrum München
2
Löıc A. Royer
Chan Zuckerberg Initiative (United States)
3
Jan-Hendrik Spille
University of Illinois Chicago
4
Smita Krishnaswamy
Yale University
5
Lin Tian
Shriners Hospitals for Children - Northern California
6
Klaus Gwosch
Carl Zeiss (Germany)
7
Yvan Eilers
Max Planck Institute for Biophysical Chemistry
8
Paul Guichard
University of Geneva
9
Jounhong Ryan Cho
California Institute of Technology
10
Guy Wolf
Université de Montréal

Papers

1
Distinct molecular profiles of skull bone marrow in health and neurological disorders
Cell|2023
158 cit.
2
DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy
Nature Methods|2022
150 cit.
3
Self-supervised deep learning encodes high-resolution features of protein subcellular localization
Nature Methods|2022
133 cit.
4
Whole-body cellular mapping in mouse using standard IgG antibodies
Nature Biotechnology|2023
121 cit.
5
Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
Nature Methods|2022
117 cit.
6
Spatial proteomics in three-dimensional intact specimens
Cell|2022
115 cit.
7
iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy
Nature Communications|2023
105 cit.
8
A multimodal zebrafish developmental atlas reveals the state-transition dynamics of late-vertebrate pluripotent axial progenitors
Cell|2024
93 cit.
9
Multiscale PHATE identifies multimodal signatures of COVID-19
Nature Biotechnology|2022
80 cit.
10
Time-series reconstruction of the molecular architecture of human centriole assembly
Cell|2024
77 cit.

Current Senior Leaders

Wrote the book, still writing it

Researchers

1
Stefan W. Hell
Max Planck Institute for Medical Research
2
Xiaowei Zhuang
Howard Hughes Medical Institute
3
Roger Y. Tsien
Howard Hughes Medical Institute
4
Rahul Satija
New York Genome Center
5
Atsushi Miyawaki
RIKEN Center for Brain Science
6
X. Sunney Xie
Peking University
7
Markus Sauer
University of Würzburg
8
Harald F. Hess
Howard Hughes Medical Institute
9
Enrico Gratton
University of California, Irvine
10
Soumya Raychaudhuri
Broad Institute

Papers

1
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
Journal of Biological Chemistry|1985
21.7k cit.
2
Comprehensive Integration of Single-Cell Data
Cell|2019
16.9k cit.
3
Integrated analysis of multimodal single-cell data
Cell|2021
16k cit.
4
Fast, sensitive and accurate integration of single-cell data with Harmony
Nature Methods|2019
10.5k cit.
5
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Science|2006
8.9k cit.
6
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
Nature Methods|2006
8.3k cit.
7
Spatial reconstruction of single-cell gene expression data
Nature Biotechnology|2015
7.7k cit.
8
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Optics Letters|1994
6.1k cit.
9
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Nature|1997
3.2k cit.
10
Spatially resolved, highly multiplexed RNA profiling in single cells
Science|2015
3.1k cit.

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