Jing Yuan

Abstract The pandemic caused by emerging coronavirus SARS-CoV-2 presents a serious global public health emergency in urgent need of prophylactic and therapeutic interventions. SARS-CoV-2 cellular entry depends on binding between the viral Spike protein receptor-binding domain (RBD) and the angiotensin converting enzyme 2 (ACE2) target cell receptor. Here, we report on the isolation and characterization of 206 RBD-specific monoclonal antibodies (mAbs) derived from single B cells of eight SARS-CoV-2 infected individuals. These mAbs come from diverse families of antibody heavy and light chains without apparent enrichment for particular families in the repertoire. In samples from one patient selected for further analyses, we found coexistence of germline and germline divergent clones. Both clone types demonstrated impressive binding and neutralizing activity against pseudovirus and live SARS-CoV-2. However, the antibody neutralizing potency is determined by competition with ACE2 receptor for RBD binding. Surprisingly, none of the SARS-CoV-2 antibodies nor the infected plasma cross-reacted with RBDs from either SARS-CoV or MERS-CoV although substantial plasma cross-reactivity to the trimeric Spike proteins from SARS-CoV and MERS-CoV was found. These results suggest that antibody response to RBDs is viral species-specific while that cross-recognition target regions outside the RBD. The specificity and neutralizing characteristics of this plasma cross-reactivity requires further investigation. Nevertheless, the diverse and potent neutralizing antibodies identified here are promising candidates for prophylactic and therapeutic SARS-CoV-2 interventions.

The genotoxicity, mutagenesis, and carcinogenic effects of polycyclic aromatic hydrocarbon (PAH) derivatives may exceed the parent PAHs. However, their influence on the soil environment has not been explored to a large extent. Oxygenated polycyclic aromatic hydrocarbons (OPAHs) and nitrated polycyclic aromatic hydrocarbons (NPAHs) are typical polar substituted compounds. We offer a review of the literature on the sources, quantification, incidence, toxicity, and transport of these compounds in soil. Although their environmental concentrations are lower than those of their parent compounds, they exert higher toxicity. Both types of substances are basically related to carcinogenesis. OPAHs are not enzymatically activated and can generate reactive oxygen species in biological cells, while NPAHs have been shown to be mutagenic, genotoxic, and cytotoxic. These compounds are largely derived from the transformation of PAHs, but they behave differently in soil because of their higher molecular weight and dissimilar adsorption mechanisms. Therefore, specialized knowledge of model derivatives is required. We also made recommendations for future directions based on existing research. It is expected that the review will trigger scientific discussions and provide a research basis for further study on PAH derivatives in the soil environment.

Heavy metal contamination poses an escalating global challenge to soil ecosystems, with hyperaccumulators playing a crucial role in environmental remediation and resource recovery. The enrichment of diazotrophs and resulting nitrogen accumulation promoted hyperaccumulator growth and facilitated phytoremediation. Nonetheless, the regulatory mechanism of hyperaccumulator biological nitrogen fixation has remained elusive. Here, we report the mechanism by which arsenic regulates biological nitrogen fixation in the arsenic-hyperaccumulator Pteris vittata. Field investigations and greenhouse experiments, based on multi-omics approaches, reveal that elevated arsenic stress induces an enrichment of key diazotrophs, enhances plant nitrogen acquisition, and thus improves plant growth. Metabolomic analysis and microfluidic experiments further demonstrate that the upregulation of specific root metabolites plays a crucial role in recruiting key diazotrophic bacteria. These findings highlight the pivotal role of nitrogen-acquisition mechanisms in the arsenic hyperaccumulation of Pteris vittata, and provide valuable insights into the plant stress resistance. Elevated arsenic is found to enhance plant nitrogen acquisition and plant growth of the arsenic hyperaccumulator Pteris vittate. Multi-omics analysis reveals the interaction between root metabolites and key diazotrophs underlying this effect.

Constructing an ecological security pattern is vital to guaranteeing regional ecological security. The terrain and geomorphology of the alpine valley are complex and sensitive, meaning it is difficult to construct ecological security patterns. Therefore, the study takes Nujiang Prefecture as the study area and builds an “Importance–Sensitivity–Connectivity” (Importance of ecosystem service, eco-environmental sensitivity, and landscape connectivity) framework to carry on the comprehensive evaluation of the ecological security and identification of ecological sources. Furthermore, we constructed an ecological resistance surface using land-use type. Using the minimum cumulative resistance (MCR) model, the study identifies the ecological corridors and nodes to build ecological security patterns to optimize the ecological spatial structure of Nujiang Prefecture. The results showed that (1) the importance of ecosystem services was higher in the west and lower in the east. The high-sensitive areas of the ecological environment were distributed discontinuously along the banks of the Nujiang and the Lantsang River, and the areas with high landscape connectivity were distributed in patches in the Gaoligong Mountain Nature Reserve and the Biluo Snow Mountain. (2) The overall ecological security was in a good state, and the ecologically insecure areas were primarily distributed in Lanping County and the southeast region of Lushui City. (3) The primary ecological source area was identified to be 3281.35 km2 and the secondary ecological source area to be 4224.64 km2. (4) In total, 26 primary ecological corridors, 39 secondary ecological corridors, and 82 ecological nodes were identified.