R.E. Brown

A low molecular weight inhibitor of cell-free protein synthesis effective at subnanomolar concentrations is formed on incubation of cytoplasmic extracts from interferon-treated cells with double-stranded RNA and ATP. It can be conveniently synthesized by incubating a poly(I).poly(C)-Sepharose-bound enzyme fraction from such cells with [3H]- or [alpha- or gamma-32P]ATP. The radioactive inhibitor has been characterized by its behavior on DEAE-Sephadex in the presence of urea and on the basis of the products obtained on enzymic, alkaline, and sequential degradation by periodate oxidation and beta elimination. Its structure appears to be pppA2'p5'A2'p5'A. We have found no evidence for any modification or abnormality other than the 2'-5' linkage. On occasion the inhibitor preparations have included what seems to be the corresponding dimer (pppA2'p5'A), tetramer [ppp(A2'p)3A], pentamer [ppp(A2'p)4A], and higher oligomers in decreasing amounts. The trimer, tetramer, and pentamer are similar in activity, but the dimer is less potent if active at all.

Journal Article Faith and Works: Church-Based Social Capital Resources and African American Political Activism Get access R. Khari Brown, R. Khari Brown University of Michigan Direct correspondence to R. Khari Brown, Program for Research on Black Americans, 5062 Institute for Social Research, University of Michigan, Ann Arbor, MI 48106-1248. E-mail: kharib@umich.edu. Search for other works by this author on: Oxford Academic Google Scholar Ronald E. Brown Ronald E. Brown Wayne State University Search for other works by this author on: Oxford Academic Google Scholar Social Forces, Volume 82, Issue 2, December 2003, Pages 617–641, https://doi.org/10.1353/sof.2004.0005 Published: 01 December 2003