J G McCormack

Many hormones and other external stimuli influence intramitochondrial metabolism (25). The mechanisms by which this occurs involve not only signal transmission across the plasma membrane but also across the inner membrane of mitochondria. This latter membrane is essentially impermeable to all charged molecules unless a specific carrier or transport system is present in the membrane. Of the known second-messenger molecules that act within the cytosolic compartment of mammalian cells, it appears that only Ca2+ is transferred into the mitochondrial matrix. The Ca2+ -transport system in the inner membrane of mitochondria (Figure 1) has separate uptake and efflux components (for reviews see 9, 14, 70). Uptake of Ca2+ occurs via an electrophoretic uniporter driven by the large electrical potential across the inner membrane. This process is inhibited by the dye ruthenium red and also by Mg2+ at concentrations likely to be present in the cytoplasm of cells (i.e. about 1 mM). The transfer of Ca2+ out of the mitochondria in heart and many other tissues occurs mainly via an e1ec-

1. In uncoupled rat heart mitochondria, the kinetic parameters for oxoglutarate oxidation were very close to those found for oxoglutarate dehydrogenase activity in extracts of the mitochondria. In particular, Ca2+ greatly diminished the Km for oxoglutarate and the k0.5 value (concentration required for half-maximal effect) for this effect of Ca2+ was close to 1 microM. 2. In coupled rat heart mitochondria incubated with ADP, increases in the extramitochondrial concentration of Ca2+ greatly stimulated oxoglutarate oxidation at low concentrations of oxoglutarate, but not at saturating concentrations of oxoglutarate. The k0.5 value for the activation by extramitochondrial Ca2+ was about 20 nM. In the presence of either Mg2+ or Na+ this value was increased to about 90 nM, and in the presence of both to about 325 nM. 3. In coupled rat heart mitochondria incubated without ADP, increases in the extramitochondrial concentration of Ca2+ resulted in increases in the proportion of pyruvate dehydrogenase in its active non-phosphorylated form. The sensitivity to Ca2+ closely matched that found to affect oxoglutarate oxidation, and Mg2+ and Na+ gave similar effects. 4. Studies of others have indicated that the distribution of Ca2+ across the inner membrane of heart mitochondria is determined by a Ca2+-transporting system which is composed of a separate uptake component (inhibited by Mg2+ and Ruthenium Red) and an efflux component (stimulated by Na+). The present studies are entirely consistent with this view. They also indicate that the intramitochondrial concentration of Ca2+ within heart cells is probably about 2--3 times that in the cytoplasm, and thus the regulation of these intramitochondrial enzymes by Ca2+ is of likely physiological significance. It is suggested that the Ca2+-transporting system in heart mitochondria may be primarily concerned with the regulation of mitochondrial Ca2+ rather than cytoplasmic Ca2+; the possible role of Ca2+ as a mediator of the effects of hormones and neurotransmitters on mammalian mitochondrial oxidative metabolism is discussed.

Three key dehydrogenases in mammalian mitochondria have been found to be activated by Ca2+ with a half-maximal effect at approximately 1 microM. These are pyruvate dehydrogenase, NAD+-isocitrate dehydrogenase, and oxoglutarate dehydrogenase. Activation of these enzymes can also be demonstrated in intact coupled mitochondria when extra mitochondrial Ca2+ is increased in the range of concentrations (0.1 to 2 microM) generally considered to occur in the cytoplasm of normal cells. It is argued that the main role of the calcium transport system in mammalian mitochondria is to relay changes in cytoplasmic Ca2+ into the mitochondrial matrix. Hormones and other extracellular messengers which stimulate ATP-requiring processes such as secretion or muscle contraction through increasing the cytoplasmic concentration of Ca2+ could in this way also increase intramitochondrial oxidative metabolism and hence promote the replenishment of ATP. Recent evidence obtained with heart and liver preparations in support of this view is reviewed.

1. Increasing concentrations of both Ca2+ and Sr2+ (generated by using EGTA buffers) resulted in 4-fold increases in the initial activity of pyruvate dehydrogenase within intact uncoupled mitochondria from rat epididymal adipose tissue incubated in the presence of the ionophore A23187, ATP, Mg2+ and oligomycin. The k0.5 values (concentrations required for half-maximal effects) for Ca2+ and Sr2+ were 0.54 and 7.1 microM respectively. In extracts of the mitochondria, pyruvate dehydrogenase phosphate phosphatase activity was stimulated about 4-fold by Ca2+ and Sr2+, with k0.5 values of 1.08 and 6.4 microM respectively. 2. NAD+-isocitrate dehydrogenase and oxoglutarate dehydrogenase appeared to be rate-limiting in the oxidation of threo-Ds-isocitrate and oxoglutarate by uncoupled mitochondria from brown adipose tissue of cold-adapted rats. Ca2+ (and Sr2+) diminished the Km for the oxidation of both threo-Ds-isocitrate and oxoglutarate. The kinetic constants for these oxidations were very similar to those obtained for the activities of NAD+-isocitrate dehydrogenase and oxoglutarate dehydrogenase in extracts of the mitochondria. In particular, the k0.5 values for Ca2+ were all in the range 0.2--1.6 microM and Sr2+ was found to mimic Ca2+, but with k0.5 values about 10 times greater. 3. Overall, the results of this study demonstrate that the activities of pyruvate dehydrogenase, NAD+-isocitrate dehydrogenase and oxoglutarate dehydrogenase may all be increased by Ca2+ and Sr2+ within intact mitochondria. In all cases the k0.5 values are close to 1 and 10 microM respectively, as found for the separated enzymes. Experiments on brown-adipose-tissue mitochondria incubated in the presence of albumin suggest that it may be possible to use the sensitivity of the dehydrogenases to Ca2+ as a means of assessing the distribution of Ca2+ across the mitochondrial inner membrane.