The Use and Misuse of FTIR Spectroscopy in the Determination of Protein StructureMichael Jackson, Henry H. Mantsch|Critical Reviews in Biochemistry and Molecular Biology|1995 Fourier transform infrared (FTIR) spectroscopy is an established tool for the structural characterization of proteins. However, many potential pitfalls exist for the unwary investigator. In this review we critically assess the application of FTIR spectroscopy to the determination of protein structure by (1) outlining the principles underlying protein secondary structure determination by FTIR spectroscopy, (2) highlighting the situations in which FTIR spectroscopy should be considered the technique of choice, (3) discussing the manner in which experiments should be conducted to derive as much physiologically relevant information as possible, and (4) outlining current methods for the determination of secondary structure from infrared spectra of proteins.
Fourier Self-Deconvolution: A Method for Resolving Intrinsically Overlapped BandsThe general theory of Fourier self-deconvolution, i.e., spectral deconvolution using Fourier transforms and the intrinsic lineshape, is developed. The method provides a way of computationally resolving overlapped lines that can not be instrumentally resolved due to their intrinsic linewidth. Examples of the application of the technique to synthetic and experimental infrared spectra are presented, and potential applications are discussed. It is shown that lines in spectra having moderate signal/noise ratios (∼1000) can readily be reduced in width by a factor of 3. The method is applicable to a variety of spectroscopic techniques.
Infrared spectroscopy of biomoleculesTheoretical Analyses of the Amide I Infrared Bands of Globular Proteins (H. Torii & M. Tasumi). Fourier Transform Infrared Spectroscopy of Enzyme Systems (J. Alben). Light--Induced Fourier Transform Infrared Difference Spectroscopy of the Primary Electron Donor in Photosynthetic Reaction Centers (E. Nabedryk). Equipment: Slow and Fast Infrared Kinetic Studies (F. Siebert). Ultrafast Infrared Spectroscopy of Biomolecules (B. Cohen & R. Hochstrasser). Infrared Spectroscopy of Nucleic Acids (J. Liquier & E. Taillandier). Fourier Transform Infrared Spectroscopy in the Study of Hydrated Lipids and Lipid Bilayer Membranes (R. Lewis & R. McElhaney). Fourier Transform Infrared Spectroscopic Studies of Cell Surface Polysaccharides (K. Brandenburg & U. Seydel). Fourier Transform Infrared Spectroscopy of Biomembrane Systems (P. Haris & D. Chapman). What Can Infrared Spectroscopy Tell Us About the Structure and Composition of Intact Bacterial Cells? (D. Naumann, et al.). Biomedical Infrared Spectroscopy (M. Jackson & H. Mantsch). New Trends in Isotope--Edited Infrared Spectroscopy (H. Fabian, et al.). Index.
Beware of connective tissue proteins: Assignment and implications of collagen absorptions in infrared spectra of human tissuesMichael Jackson, Lin-P'ing Choo, Peter H. Watson et al.|Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease|1995 Fourier transforms in the computation of self-deconvoluted and first-order derivative spectra of overlapped band contoursADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFourier transforms in the computation of self-deconvoluted and first-order derivative spectra of overlapped band contoursJyrki K. Kauppinen, Douglas J. Moffatt, Henry H. Mantsch, and David G. CameronCite this: Anal. Chem. 1981, 53, 9, 1454–1457Publication Date (Print):August 1, 1981Publication History Published online1 May 2002Published inissue 1 August 1981https://pubs.acs.org/doi/10.1021/ac00232a034https://doi.org/10.1021/ac00232a034research-articleACS PublicationsRequest reuse permissionsArticle Views1086Altmetric-Citations295LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts