George Brecher

Journal Article The Reproducibility and Constancy of the Platelet Count Get access George Brecher, M.D., George Brecher, M.D. National Institute for Arthritis and Metabolic Diseases and the National Cancer Institute, National Institutes of Health, U.S. Public Health Service, and the Naval Medical Research Institute, Bethesda, Maryland Search for other works by this author on: Oxford Academic Google Scholar Marvin Schneiderman, B.S., Marvin Schneiderman, B.S. National Institute for Arthritis and Metabolic Diseases and the National Cancer Institute, National Institutes of Health, U.S. Public Health Service, and the Naval Medical Research Institute, Bethesda, Maryland Search for other works by this author on: Oxford Academic Google Scholar Eugene P. Cronkite, M.D. Eugene P. Cronkite, M.D. National Institute for Arthritis and Metabolic Diseases and the National Cancer Institute, National Institutes of Health, U.S. Public Health Service, and the Naval Medical Research Institute, Bethesda, Maryland Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 23, Issue 1, 1 January 1953, Pages 15–26, https://doi.org/10.1093/ajcp/23.1.15 Published: 01 January 1953 Article history Received: 26 September 1952 Published: 01 January 1953

Journal Article Platelet Counts with the Coulter Counter Get access B. S. Bull, M.D., B. S. Bull, M.D. Clinical Pathology Department, Clinical Center and Biometry Branch, National Cancer Institute, National Institutes of Health, U. S. Public Health Service, Bethesda, Maryland 20014 Search for other works by this author on: Oxford Academic Google Scholar M. A. Schneiderman, PH.D., M. A. Schneiderman, PH.D. Clinical Pathology Department, Clinical Center and Biometry Branch, National Cancer Institute, National Institutes of Health, U. S. Public Health Service, Bethesda, Maryland 20014 Search for other works by this author on: Oxford Academic Google Scholar George Brecher, M.D. George Brecher, M.D. Clinical Pathology Department, Clinical Center and Biometry Branch, National Cancer Institute, National Institutes of Health, U. S. Public Health Service, Bethesda, Maryland 20014 Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 44, Issue 6, 1 December 1965, Pages 678–688, https://doi.org/10.1093/ajcp/44.6.678 Published: 01 December 1965 Article history Received: 20 May 1965 Published: 01 December 1965

Abstract Thirteen patients with chronic myelogenous leukemia continued to have the Ph1 chromosomes in 90-100 per cent dividing marrow cells during drug-induced clinical remissions. The Ph1 chromosome was present in erythroid as well as granulocytic marrow cells, and possibly in megakaryocytes. The presence of Ph1 chromosomes was also studied in cultures of peripheral blood. In six patients in relapse, 40 per cent of metaphases contained the Ph1 chromosome, and the percentage of these cells corresponded roughly to the relative frequency of immature granulocytes in the blood. In contrast, during remission, few or no Ph1 chromosomes were found in peripheral blood cultures, presumably because in the absence of immature granulocytes the dividing cells in the cultures originate from lymphocytes, as they do in normal blood. It is suggested that the Ph1 chromosome usually arises in a precursor cell common to the erythroid, granulocytic, and megakaryocytic, but not the lymphoid series of hemopoietic cells.

Abstract The kinetics of growth of peripheral blood cells in tissue culture are described. There was a rapid increase of cells in DNA synthesis beginning at 24 hours and by 72 hours 40-45 per cent of the cells were in DNA synthesis. Mitotic rates of ∼ 1 per cent/hour were seen after 72 hours in cultures. Studies with tritiated thymidine and colchicine indicated that these cells represent transformed lymphocytes and do not arise from the cells in DNA synthesis at time zero or from any other small population of cells.