Bertil Pettersson

Mycoplasma mycoides subsp. mycoidesSC (MmymySC)is the etiological agent of contagious bovine pleuropneumonia (CBPP), a highly contagious respiratory disease in cattle. The genome of Mmymy SC type strain PG1(T) has been sequenced to map all the genes and to facilitate further studies regarding the cell function of the organism and CBPP. The genome is characterized by a single circular chromosome of 1211703 bp with the lowest G+C content (24 mole%)and the highest density of insertion sequences (13% of the genome size)of all sequenced bacterial genomes. The genome contains 985 putative genes, of which 72 are part of insertion sequences and encode transposases. Anomalies in the GC-skew pattern and the presence of large repetitive sequences indicate a high genomic plasticity. A variety of potential virulence factors was identified, including genes encoding putative variable surface proteins and enzymes and transport proteins responsible for the production of hydrogen peroxide and the capsule, which is believed to have toxic effects on the animal.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical and biological characterization of organic material from gasoline exhaust particlesTomas. Alsberg, Ulf. Stenberg, Roger. Westerholm, Michael. Strandell, Ulf. Rannug, Annica. Sundvall, Lennart. Romert, Vibeke. Bernson, Bertil. Pettersson, and . et al.Cite this: Environ. Sci. Technol. 1985, 19, 1, 43–50Publication Date (Print):January 1, 1985Publication History Published online1 May 2002Published inissue 1 January 1985https://pubs.acs.org/doi/10.1021/es00131a003https://doi.org/10.1021/es00131a003research-articleACS PublicationsRequest reuse permissionsArticle Views289Altmetric-Citations109LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

Four strains of aerobic, endospore-forming bacteria were isolated from the inner tissues of healthy cotton plants (Gossypium sp., Dushanbe, Tajikistan). The organisms had identical randomly amplified polymorphic DNA patterns that distinguished them from other bacilli that are commonly isolated from plant tissues, e.g. Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus pumilus and Bacillus subtilis. PCR amplification of 16S-23S rRNA spacer regions suggested that the four strains could be assigned to two highly related taxa, which correlated with differences in cell morphology. However, the cloned spacer region provided a simple and specific hybridization probe for all four strains. The virtually complete 16S rDNA sequences were prepared for representatives of the two groups (strains 2DT(T) and 12DX) and differed by only two bases, thus supporting classification of the four strains in a single taxon at the species level. Phylogenetic analyses indicated that strain 2DT(T) belonged to the genus Bacillus and was most closely related to Bacillus sporothermodurans DSM 10599T with a sequence similarity of 94.8%. It is concluded that the four strains belong to a novel species of Bacillus for which the name Bacillus endophyticus sp. nov. is proposed. The type strain is 2DT(T) (= UCM B-5715T = CIP 106778T).

Ribosomes are present in all self-replicating cells and constitute their protein-synthesizing machinery. The ribosomes are composed of ribosomal proteins and ribosomal RNA (rRNA). Bacteria have three kinds of rRNA (5S, 16S, and 23S rRNA), and the genetic information of these molecules is organized in the genome in the form of rRNA operons. The nucleotide sequences of the rRNA molecules contain well-defined segments of different evolutionary variability, which in the 16S rRNA molecule are referred to as universal (U), semiconserved (S), and variable (V) regions (1). The universal regions are numbered U1–U8 from the 5′-terminus. A more refined model for the nucleotide substitution rates in bacterial rRNA was recently presented, and it was shown that the nucleotide substitution rates within one of the above regions can vary substantially (2). Ribosomal RNA has the same important function in the cell, irrespective of species, which means that the corresponding genes are under approximately the same evolutionary pressure. These properties together make sequence analysis of rRNA extremely suitable for phylogenetic (3) and evolutionary (4) studies.