Frank J. Triska

Chloride and nitrate were coinjected into the surface waters of a third—order stream for 20 d to examine solute retention, and the fate of nitrate during subsurface transport. A series of wells (shallow pits) 0.5—10 m from the adjacent channel were sampled to estimate the lateral interflow of water. Two subsurface return flows beneath the wetted channel were also examined. The conservative tracer (chloride) was hydrologically transported to all wells. Stream water was >88% of flow in wells <4 m from the wetted channel. The lowest percentage of stream water was 47% at a well 10 m perpendicular to the stream. Retention of solutes was greater in the hyporheic zone than in the channel under summer low—flow conditions. Nominal travel time (the interval required for chloride concentration to reach 50% of the plateau concentration) was variable by well location, indicating different flow paths and presumably permeability differences in subsurface gravels. Nominal travel time was M 24 h for wells <5 m from the wetted channel. Coinjected nitrate was not conservative. Two wells were significantly (P < .05) higher in nitrate—N than would be predicted from chloride, while four were significantly lower. Wells 2.0—4.0 m from the wetted channel tended to have higher nitrate concentration than predicted, whereas nitrate sink locations tended to have transport distances >4.3 m. The capacity of the hyporheic zone for transient solute storage and as potential biological habitat varies with channel morphology, bed roughness, and permeability. A conceptual model that considers the groundwater—stream water interface as the fluvial boundary is proposed. Emerging paradigms of the riverine network should consider the hyporheic zone and associated nutrient cycling as an integral component of fluvial structure and function.

Denitrification was assayed by the acetylene blockage technique in hyporheic sediments. Samples were obtained along transects perpendicular to the stream at two sites: (1) the base of a slope dominated by old-growth redwood and (2) the base of a slope dominated by alder regenerating from a clearcut in 1965. Denitrification was evident at in situ nitrate concentrations at all locations tested. Activity was stimulated by nitrate but nitrate plus glucose had no additional effect. Denitrifying potentials increased with increasing distance from the stream channel. Dissolved oxygen was 100% of the concentration expected in equilibrium with the atmosphere in water obtained from monitoring wells immediately adjacent to the stream but was as low as 7% of the expected value in water 11.4 m inland. Both nitrate and dissolved organic carbon decreased over summer in wells at the base of the alder-forested slope. A 48-h injection of nitrate-amended stream water into hyporheic water 8.4 m inland stimulated nitrous oxide production in the presence of acetylene. Nitrous oxide was generated as nitrate and acetylene were co-transported to a well 13 m down-gradient. The acetylene-block experiments coupled with the chemistry data suggest that denitrification can modify the chemistry of water during passage through the hyporheic zone.

A study of the wood-associated invertebrates was undertaken in seven streams of the Coast and Cascade Mountains of Oregon. The amount of wood debris was determined in terms of both weight and surface area. Standing crop of wood per unit area decreases with increasing stream order. Invertebrates associated with wood were functionally categorized and their biomass on wood determined. Major xylophagous species were the caddisfly (Heteroplectron californicum), the elmid beetle (Lora avara) and the snail (Oxytrema silicula). Standing crop of these species is greater on wood in the Coast Range than in the Cascades, which is attributed to species composition of available wood debris. The density of L. avara was strongly correlated with the amount of wood available irrespective of stream size within a drainage. The standing crop of invertebrates was about two orders of magnitude greater on leaf debris than on wood. A potential strategy for wood consumption, based on microbial conditioning, is presented. The data are used to develop a general scheme of wood processing by invertebrates in small stream ecosystems. Their impact is similar to that of invertebrates which process leaf litter in terrestrial and aquatic environments when the full decomposition cycle of wood debris is considered. INTRODUCTION The allochthonous inputs to streams in western coniferous forests include coniferous needles, deciduous leaves and woody material, ranging in size from small twigs and bark to large logs. The amount of fallen wood in these streams can be extremely large. Froehlich (1973) estimated that in one watershed of old-growth douglas fir (Pseudotsuga menziesii) the standing crop of wood debris (pieces larger than 10 cm diam) was more than 15 kg/m2. The standing crop of small debris in the same stream was 1.08 kg/m2 (Sedell et al., 1974). In view of the quantities of woody material in these streams, it is apparent that wood has a significant role in energy flow, nutrient dynamics, stream morphology and in shaping the biotic community of these lotic ecosystems. Although stream ecologists have emphasized the importance of allochthonous debris as the food base for stream invertebrates, most previous studies are based on leaf inputs (Hynes, 1970; Cummins et al., 1973; Boling et al., 1974). Current literature on aquatic invertebrate communities inhabiting logs or inundated trees has emphasized the exploitation of these sites as habitats for attachment or surfaces for grazing of periphyton (Claflin, 1968; Nilsen and Larimore, 1973; McLachlan, 1970) rather than as allochthonous energy and nutrient inputs to the aquatic system. The present study is a preliminary investigation of the wood component in coniferous stream ecosystems of western Oregon and of the role of invertebrates in the biological processing or degradation of wood. The objectives were to survey the fauna associated with wood in streams and to determine some of the interactions between the fauna and the wood substrate. In order to develop generalizations on the invertebrate-wood interactions, we chose to compare large and small streams in two different areas rather than to investigate one site in detail.

Chloride was injected as a conservative tracer with nitrate to examine nitrate retention (storage plus biotic uptake) and transport in a 327—m reach of a third—order stream draining a forested basin in northwestern California. Prior to injections, diel patterns of nutrient concentrations were measured under background conditions. Nitrate concentration of stream water increased downstream, indicating that the reach was a source of dissolved inorganic nitrogen to downstream communities under background, low—flow conditions, despite uptake by photoautotrophs. At the onset of continuous solute injection over a 10—d period, timing the passage of the solute front indicated that storage dominated nitrate retention. Instantaneous concentration differences at the base of the reach at hour 24 indicated that biotic uptake accounted for 13% of the nitrate amendment while hydrologic storage constituted 29%. Corrected for groundwater dilution (11.7%), saturation of the stream's channel and hyporheic zones was not complete until 6.8 d of continuous injection. By day 3 nitrate retention was dominated by biotic processes. Biotic uptake was greatest during daylight hours indicating retention by photoautotrophs, but also occurred during darkness. After 10 d of continuous injection, mass balance calculations indicated that 29% of N (339 g) was retained from the total injected (1155 g), while the balance of injected nitrate was transported downstream. Storage of NO3—N was 117 g or 10% while biotic uptake was 222 g or 19%. Periphyton biomass on slides, chlorophyll a both on slides and on natural cobbles, and net community primary production all indicated a lag in periphyton response to nitrate amendment. Earliest indicators of a biotic response to nutrient amendment were decreases in both tissue C/N and epilithic respiration.