CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION IAbel, K. (Melpar, Inc., Falls Church, Va.), H. deSchmertzing, and J. I. Peterson. Classification of microorganisms by analysis of chemical composition. I. Feasibility of utilizing gas chromatography. J. Bacteriol. 85:1039-1044. 1963.-The feasibility of utilizing gas chromatography as a sensitive and rapid method for the analysis of lipids as a natural basis for the classification of microorganisms by chemical composition was investigated. The lipids were extracted and transesterified to component carboxylic acid methyl esters in a single step, after which the methyl esters were resolved by gas chromatography to provide distinctive chromatographic elution patterns. Similarities in the lipid carboxylic acid distribution were noted among selected species of the family Enterobacteriaceae, and significant differences were noted among selected families of the class Schizomycetes.
ANALYSIS OF CRYOGENTICALLY TRAPPED TRACE CONTAMINANTS BY IONIZING GAS CHROMATOGRAPHY.Abstract : The purpose of the work was to determine quantitatively the concentration of micro contaminants in a sealed environmental system. The separation and identification of the cryogenically trapped trace contaminants was accomplished with liquid gas chromatography using a flame ionization detector. Forty sets of samples were analyzed, each set consisting of 3 cylinders, trapped at Brooks AFB. The compounds contained in the sample cylinders were identified by their elution time from a column and the amount measured with the aid of peak areas. The response characteristics of the chromatograph were calculated from response obtained with standard mixtures. The forty sample sets were composed of 9 samples from a manned simulator run conducted in October-November, 1965; 30 samples from a similar run conducted in March-April 1966, and one sample set consisting of trapping efficiency runs. (Author)