H. Sternlicht

A role in folding newly translated cytoskeletal proteins in the cytosol of eukaryotes has been proposed for t-complex polypeptide 1 (TCP1). In this study, we investigated tubulin and actin biogenesis in Chinese hamster ovary (CHO) cells. When extracts of pulse-labeled cells were analyzed by anion-exchange and size-exclusion chromatography, newly synthesized alpha-tubulin, beta-tubulin, and actin were observed to enter a large molecular mass complex (approximately 900 kDa). These proteins were released from this complex capable, in the case of tubulin, of forming heterodimers. The large molecular mass complexes coeluted with TCP1 and could be immunoprecipitated by using an anti-TCP1 antibody. These findings demonstrate that there is a cytosolic pathway for folding tubulin and actin in vivo that involves the TCP1 complex.

This paper deals with triplet—triplet annihilation in pure and mixed organic crystals. In crystals containing a small concentration of impurity traps, triplet excitation migration may proceed from trap to trap on a time scale which is short compared with the long triplet state lifetime but which is long compared with the normal fluorescence lifetime. Nearest-neighbor and long-range mutual annihilation of two triplets may then take place giving rise to delayed fluorescence. The rates of long-range triplet excitation migration and annihilation show a concentration dependence, a temperature dependence, and a solvent dependence. Providing the triplet—triplet annihilation rate is not too fast, the intensity of the delayed fluorescence can be shown to depend upon the square of the intensity of the exciting light. This expectation is borne out by experiments, briefly reported here, on delayed fluorescence in dilute isotopic mixed crystals. In crystals containing high concentrations of such impurity traps, or in pure crystals, the annihilation rate becomes extremely rapid and this mechanism effectively quenches phosphorescence in many, but not all, classes of pure organic crystals. The kinetics of the over-all process are discussed in both the limits of fast and slow annihilation rates. A theoretical investigation of the origin of the annihilation matrix element is carried out, and it is shown that exchange interactions play the largest role in determining annihilation rates. Past work [H. Sponer, Y. Kanda, and L. A. Blackwell, J. Chem. Phys. 29, 721 (1958); N. W. Blake and D. S. McClure, ibid., p. 722] on delayed fluorescence of assumed pure naphthalene crystals containing very small amounts of β-methyl naphthalene (traps) can be understood within the framework of this paper. The need in organic crystals for the major delayed fluorescence mechanism to be based upon ionization and electron trapping seems now to be considerably lessened.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-13 magnetic resonance studies of amino acids and peptidesW. J. Horsley and H. SternlichtCite this: J. Am. Chem. Soc. 1968, 90, 14, 3738–3748Publication Date (Print):July 1, 1968Publication History Published online1 May 2002Published inissue 1 July 1968https://pubs.acs.org/doi/10.1021/ja01016a025https://doi.org/10.1021/ja01016a025research-articleACS PublicationsRequest reuse permissionsArticle Views225Altmetric-Citations166LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-13 magnetic resonance studies of amino acids and peptides. IIW. J. Horsley, H. Sternlicht, and Jack Sidney CohenCite this: J. Am. Chem. Soc. 1970, 92, 3, 680–686Publication Date (Print):February 1, 1970Publication History Published online1 May 2002Published inissue 1 February 1970https://pubs.acs.org/doi/10.1021/ja00706a042https://doi.org/10.1021/ja00706a042research-articleACS PublicationsRequest reuse permissionsArticle Views210Altmetric-Citations167LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts