Noémie Danné

In recent years, exploration of the brain extracellular space (ECS) has made remarkable progress, including nanoscopic characterizations. However, whether ECS precise conformation is altered during brain pathology remains unknown. Here we study the nanoscale organization of pathological ECS in adult mice under degenerative conditions. Using electron microscopy in cryofixed tissue and single nanotube tracking in live brain slices combined with super-resolution imaging analysis, we find enlarged ECS dimensions and increased nanoscale diffusion after α-synuclein-induced neurodegeneration. These animals display a degraded hyaluronan matrix in areas close to reactive microglia. Furthermore, experimental hyaluronan depletion in vivo reduces dopaminergic cell loss and α-synuclein load, induces microgliosis and increases ECS diffusivity, highlighting hyaluronan as diffusional barrier and local tissue organizer. These findings demonstrate the interplay of ECS, extracellular matrix and glia in pathology, unraveling ECS features relevant for the α-synuclein propagation hypothesis and suggesting matrix manipulation as a disease-modifying strategy.

The intrinsic near-infrared photoluminescence observed in long single-walled carbon nanotubes is known to be quenched in ultrashort nanotubes due to their tiny size as compared to the exciton diffusion length in these materials (>100 nm). Here, we show that intense photoluminescence can be created in ultrashort nanotubes (∼40 nm length) upon incorporation of exciton-trapping sp3 defect sites. Using super-resolution photoluminescence imaging at <25 nm resolution, we directly show the preferential localization of excitons at the nanotube ends, which separate by less than 40 nm and behave as independent emitters. This unexpected observation opens the possibility to synthesize fluorescent ultrashort nanotubes—a goal that has been long thought impossible—for bioimaging applications, where bright near-infrared photoluminescence and small size are highly desirable, and for quantum information science, where high quality and well-controlled near-infrared single photon emitters are needed.

A micrometer-sized spherical particle classically equilibrates at the water-air interface in partial wetting configuration, causing about no deformation to the interface. In condition of thermal equilibrium, the particle just undergoes faint Brownian motion, well visible under a microscope. We report experimental observations when the particle is made of a light-absorbing material and is heated up by a vertical laser beam. We show that, at small laser power, the particle is trapped in on-axis configuration, similarly to 2-dimensional trapping of a transparent sphere by optical forces. Conversely, on-axis trapping becomes unstable at higher power. The particle escapes off the laser axis and starts orbiting around the axis. We show that the laser-heated particle behaves as a microswimmer with velocities on the order of several 100 μm/s with just a few milliwatts of laser power.

Luminescent single-walled carbon nanotubes (SWCNTs) are unique nanoemitters that allow near-infrared single-molecule detection within biological tissues. Interestingly, the recent discovery of upconversion luminescence from (6,5) SWCNTs provides a novel opportunity for deep tissue single SWCNT detection. Yet, the optimal excitation strategy for video-rate imaging of individual SWCNTs within live tissues needs to be determined taking into account the constraints imposed by the biological matter. Here, we directly compare the luminescence efficiencies of single (6,5) SWCNTs excited by continuous-wave lasers at their second-order excitonic transition, at their K-momentum exciton–phonon sideband, or through upconversion. For these three excitations spanning visible to near-infrared wavelengths, the relevance of single SWCNT imaging is considered inside brain tissue. The effects of tissue scattering, absorption, autofluorescence, and temperature increase induced by excitation light are systematically examined.