Candice E. Paulsen

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTCysteine-Mediated Redox Signaling: Chemistry, Biology, and Tools for DiscoveryCandice E. Paulsen and Kate S. Carroll*View Author Information Department of Chemistry, The Scripps Research Institute, Jupiter, Florida, 33458, United States*Address: The Scripps Research Institute 130 Scripps Way, #2B2 Jupiter, FL 33458. E-mail: [email protected]. Phone: (561)-228-2460. Fax: (561) 228-2919.Cite this: Chem. Rev. 2013, 113, 7, 4633–4679Publication Date (Web):March 20, 2013Publication History Received19 April 2012Published online20 March 2013Published inissue 10 July 2013https://doi.org/10.1021/cr300163eCopyright © 2013 American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceArticle Views25902Altmetric-Citations744LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (12 MB) Get e-AlertsSUBJECTS:Disulfides,Modification,Monomers,Peptides and proteins,Thiols Get e-Alerts

Hydrogen peroxide (H(2)O(2)) acts as a second messenger that can mediate intracellular signal transduction via chemoselective oxidation of cysteine residues in signaling proteins. This Review presents current mechanistic insights into signal-mediated H(2)O(2) production and highlights recent advances in methods to detect reactive oxygen species (ROS) and cysteine oxidation both in vitro and in cells. Selected examples from the recent literature are used to illustrate the diverse mechanisms by which H(2)O(2) can regulate protein function. The continued development of methods to detect and quantify discrete cysteine oxoforms should further our mechanistic understanding of redox regulation of protein function and may lead to the development of new therapeutic strategies.