Cited by 340
National Institutes of Health
Publishes on RNA Research and Splicing, RNA modifications and cancer, MicroRNA in disease regulation. 6 papers and 679 citations.
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Significance Small RNA-guided gene-silencing pathways regulate fundamental cellular processes. Small RNAs such as microRNAs (miRNAs) directly bind to a member of the Argonaute (Ago) protein family. In animals, Ago proteins interact with a member of the GW protein family (referred to as TNRC6A-C). Based on an Ago-interacting TNRC6 peptide, we have developed a method allowing for the efficient isolation and characterization of Ago protein complexes from any animal organism. We refer to this method as “Ago protein Affinity Purification by Peptides.” Our approach also allows for the identification of Ago-bound small RNAs as well as mRNAs. Expression of this peptide in living cells leads to global miRNA inactivation, thus providing a powerful tool to study miRNA function on various levels.