Isabella De Meo

In participatory forest planning, relevant stakeholders are included in the decision-making process. Stakeholder analysis is a crucial step in the participatory process in order to involve all groups of interests, increasing the legitimacy and transparency of the process. On the other hand, to gather an active stakeholder group that can communicate effectively, the number of key stakeholders should be restricted. This study aims to devise a nonsubjective method to identify and classify stakeholders in three categories (key, primary, and secondary stakeholders), taking into account the relationships among them (social network analysis). Stakeholders are classified according to the regular equivalence and betweenness centrality. The stakeholders' classification system is tested in two forest landscape management plans in Italy. Results show that the method is able to balance stakeholders' contributions and can be used to enhance the democratization of the decision-making process when power is unevenly distributed among the stakeholders.

As soil fungi are major players in the carbon accumulation process, the two main fungal degraders in topsoil, Ascomycetes and Basidiomycetes, were investigated as indicators of land use effectiveness in increasing soil carbon accumulation and soil function. The study focused on the soil organic carbon content increase in a 20-year short rotation forest cycle with broadleaf woody plantations compared to a nearby arable cropping system. Total fungi, Ascomycota and Basidiomycota, were quantified in terms of DNA copy number, with specific probes using SYBR® Green I dye on the QuantStudio™ 3D digital PCR system (dPCR). Previously, next generation sequencing analysis using a general primer confirmed that Ascomycota and Basidiomycota were the most represented phyla and that fungal community composition significantly differed between treatments. A range of key soil enzyme activities for the C-cycle were also assessed. Total organic carbon content (TOC), microbial biomass in term of dsDNA and enzyme activities significantly increased in woody plantations compared to arable soil. The TOC increase differed significantly also between wood species, Salix and Robina gave the greatest increase (+30 and 20 % respectively), followed by Populus (+12), microbial biomass highly correlated with TOC showing the same trend. Total fungi, Ascomycota and Basidiomycota increased significantly in three woody plantations compared to the arable soil system. Ascomycota in the woody plantations increased two to four times (average 3144 n copies µl−1 of DNA) compared to the arable soil (1419 n copies µl−1); Basidiomycota were almost absent in arable soil (av. 94n copies µl−1) and increased five to six times in woody plantations (av. 490 n copies µl−1). Total fungi and ascomycetes correlated strongly with microbial biomass and TOC, whereas basidiomycetes did not. These findings showed that Ascomycota represent the largest portion of fungi in agricultural soils even after a 20-years short rotation forest cycle and they can be taken as indicators of carbon accumulation processes. Therefore, this study suggests that joining the Ascomycetes quantity in the topsoil to the Ascomycota:Basidiomycota ratio seems a good option when setting regional strategies for improving C accrual in farmland with the short-term afforestation.

Provision of forest ecosystem services is influenced by site and stand characteristics as well as forest management practices. In order to evaluate the influence of forest management on ecosystem services provision, two artificial black pine forests located in Central Italy were studied where two different thinning approaches (traditional and selective) were applied under the SelPiBio LIFE project. Four main ecosystem services were selected and assessed: timber and bioenergy production, carbon sequestration, forest stand stability-protection, and biodiversity conservation. Even if not supported by statistical evidence, results highlighted an interesting trend just 2 years after treatment. The selective thinning was able to enhance the majority of ecosystem services compared to the traditional one. A higher growth rate of selected crop trees was measured (i.e., carbon sequestration). The slenderness ratio was sensibly reduced (i.e., mechanical stability) with a positive implication on soil retention and the prevention of landslides. Moreover, valuable and interesting commercial assortments have been proven to be retrieved from the stands with the selective approach. Larger and also better formed trees were harvested, given the impact of selective thinning on the co-dominant class. The Shannon index increased only with the selective thinning intervention. In conclusion, the provided results and methods are encouraging and might represent the basis for novel and longer monitoring efforts.