Donna C. Bumpass

The ETS domain transcription factor Elk-1 serves as an integration point for different mitogen-activated protein (MAP) kinase pathways. Phosphorylation of Elk-1 by MAP kinases triggers its activation. However, while the activation process is well understood, its downregulation-inactivation is less well characterized. The ETS DNA-binding domain plays a role in the downregulation of Elk-dependent promoter activity following mitogenic activation by recruiting the mSin3A-HDAC complex. Here we have identified a novel evolutionarily conserved repression domain in Elk-1, termed the R motif, which serves to reduce the basal transcriptional activity of Elk-1 and dampen its response to mitogenic signals. This domain is highly potent and portable and can repress transcription in trans. The R motif is related to the CRD1 repression domain in p300 and can functionally replace this domain and confer p21(waf1/cip1) inducibility on p300. However, the R motif acts in a context-dependent manner and is not p21(waf1/cip1) responsive in Elk-1. Thus, the Elk-1 R motif and the p300 CRD1 motif represent a new class of repression domains that are regulated in a context-dependent manner.

Conference Abstract| October 01 2001 Regulation of p300/CBP activity by the CDK inhibitor p21WAF1/CIP1 D.C. Bumpass; D.C. Bumpass 1Wellcome Trust Biocentre, University of Dundee, School of Life Sciences, MSI/WTB Complex, Dow Street, Dundee, DD1 5EH, UK Search for other works by this author on: This Site PubMed Google Scholar A. Thain; A. Thain 1Wellcome Trust Biocentre, University of Dundee, School of Life Sciences, MSI/WTB Complex, Dow Street, Dundee, DD1 5EH, UK Search for other works by this author on: This Site PubMed Google Scholar D.J. Gregory; D.J. Gregory 1Wellcome Trust Biocentre, University of Dundee, School of Life Sciences, MSI/WTB Complex, Dow Street, Dundee, DD1 5EH, UK Search for other works by this author on: This Site PubMed Google Scholar N.D. Perkins N.D. Perkins 1Wellcome Trust Biocentre, University of Dundee, School of Life Sciences, MSI/WTB Complex, Dow Street, Dundee, DD1 5EH, UK Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (2001) 29 (5): A113. https://doi.org/10.1042/bst029a113c Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation D.C. Bumpass, A. Thain, D.J. Gregory, N.D. Perkins; Regulation of p300/CBP activity by the CDK inhibitor p21WAF1/CIP1. Biochem Soc Trans 1 October 2001; 29 (5): A113. doi: https://doi.org/10.1042/bst029a113c Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 2001 Biochemical Society2001 Article PDF first page preview Close Modal You do not currently have access to this content.