Vector for High-Throughput Sequencing: Construction and Preparation with Cyclic Cut-Ligation

Abstract

Figure 2. Agarose gel electrophoresis of the modified vector DNA prepared with cyclic cutligation. M, DNA/ Hin dIII; lane 1, pSV-20 cut with Kpn I and Sac I; lane 2, pSV-20 prepared with cyclic cut-ligation reaction (see text for details); lane 3, the isolated 2.8-kb vector fragment; lane 4, no ligation was detected between the isolated vector fragments prepared with cyclic cut-ligation.