Additional file 9: Figure S5. of Positive geotactic behaviors induced by geomagnetic field in Drosophila

Abstract

CRY and Pyx function in JO for the near-zero GMF-induced negative geotaxis. (A, C, E) Comparisons of the geotactic positioning of CRY-, Pyx-, and PDF-deficient flies in which the expression of CRY, Pyx, and PDF was genetically restored in JO using nan-GAL4, a JO-specific GAL4 driver. Error bars: SEM. n.s., not significant. *, P < 0.05; **, P < 0.01; ***, P < 0.005, ****, P < 0.001 by Student’s t-test. (B, D, F) Comparisons of the geotactic positioning of CRY-, Pyx-, and PDF-deficient flies in which the expression of CRY, Pyx, and PDF was genetically restored using another JO-specific GAL4 driver, iav-GAL4. Error bars: SEM. n.s., not significant. **, P < 0.01; ***, P < 0.005 by Student’s t-test. (G, H) Comparisons of the geotactic positioning by wild-type flies, control flies (GAL4 driver alone, UAS-pdf RNAi alone), and the flies with RNAi knockdown of pdf using cry-GAL4 or pyx-GAL4 driver. Error bars: SEM. n.s.: not significant. **, P < 0.01; ***, P < 0.005; ****, P < 0.001 by Student’s t-test. For all the data, n = 10 trials. (PDF 122 kb)