RVI-seq - Multi-respiratory virus sequencing protocol v1
Andrew Wong, Ben Hyatt(Wellcome Sanger Institute), Diana Rajan(Wellcome Sanger Institute), Sarah F. Field(Wellcome Sanger Institute), Emma Dawson(Wellcome Sanger Institute), Tobi Ajenifuja(Wellcome Sanger Institute), Scott Thurston(Wellcome Sanger Institute), Katherine L. Bellis(Wellcome Sanger Institute), Josef Wagner(Wellcome Sanger Institute), Matthew Sinnott(Wellcome Sanger Institute), Andrea Frick-Kretschmer(Wellcome Sanger Institute), Frank Schwach(Wellcome Sanger Institute), Florent Lassalle(Wellcome Sanger Institute), David K. Jackson(Wellcome Sanger Institute), Sara Stott(Wellcome Sanger Institute), Ian Johnston(Wellcome Sanger Institute), Ewan M. Harrison(Wellcome Sanger Institute)
Cited by 1Open Access
Abstract
RVI-seq is a protocol for sequencing a range of clinically important respiratory viruses in a single workflow. This SOP describes the procedure for generating Illumina compatible libraries from total nucleic acid extracted from upper respiratory tract swabs (nose and throat). Up to 200ng of each library is combined in 8-plex hyb pools prior to enrichment with the Twist Respiratory Viral Research Panel. Captured sequences are cleaned up and further amplified before sequencing on the Illumina NovaSeq 6000.
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