Additional file 2 of Efficient and versatile multiplex prime editing in hexaploid wheat
Pei Ni(China Agricultural University), Yidi Zhao(China Agricultural University), Ximeng Zhou(China Agricultural University), Zehua Liu(China Agricultural University), Zhengwei Huang(China Three Gorges University), Zhongfu Ni(China Agricultural University), Qixin Sun(China Agricultural University), Yuan Zong(Chinese Academy of Sciences)
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Abstract
Additional file 2: Table S1. pegRNA target sites, RT templates and PBS sequences. Table S2. Summary of genotypes of individual plants induced by CMPE-ePPEplus in wheat plants in T0 generation. Table S3. Summary of mutations in each targeted gene induced by CMPE-ePPEplus in wheat plants in T0 generation. Table S4. Summary of simultaneous editing of multiple genes induced by CMPE-ePPEplus in wheat plants in T0 generation. Table S5. Analysis of potential off-target effects in regenerated wheat plants. Table S6. Segregation and transegene-free analysis of six T0 lines drived from CMPE-ePPEplus [63]. Table S7. PCR primers used in this study.
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