Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age
Collyn M. Kellogg(Oklahoma Medical Research Foundation), Kevin D. Pham(Oklahoma Medical Research Foundation), Sung-Hwan Ko(Oklahoma Medical Research Foundation), Jillian E. J. Cox(Oklahoma Medical Research Foundation), Adeline H. Machalinski(Oklahoma Medical Research Foundation), Michael B. Stout(Oklahoma Medical Research Foundation), Amanda L. Sharpe(University of Oklahoma Health Sciences Center), Michael J. Beckstead(Oklahoma Medical Research Foundation), Ana J. Chucair‐Elliott(Oklahoma Medical Research Foundation), Sarah R. Ocañas(Oklahoma Medical Research Foundation), Willard M. Freeman(Oklahoma Medical Research Foundation)
Cited by 4Open Access
Abstract
mice were crossed to a floxed NuTRAP reporter to compare Cre induction at early (3-6 m.o.) and late (20 m.o.) ages. Specificity and efficiency of microglial labeling at 21-22 m.o. were identical in mice induced with Tam at early and late ages. Age-related microglial translatomic changes were also similar regardless of Tam induction age. Each Cre and flox mouse line should be independently validated, however, these findings demonstrate that Tam-mediated Cre induction can be performed even into older mouse ages and should be generalizable to other inducible Cre models.
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