Serialized On-grid Lift-In Sectioning for Tomography (SOLIST)
Dung NHT(Human Technopole), G Perone(Human Technopole), Roberta Vazzana(Human Technopole), Flaminia Kaluthantrige Don(Human Technopole), Malan Silva(Human Technopole), Simona Sorrentino(Human Technopole), Paolo Swuec(Human Technopole), Frédéric R. Leroux(Vistec Electron Beam (Germany)), Nereo Kalebic(Human Technopole), Francesca Coscia(Human Technopole), Philipp S. Erdmann(Human Technopole)
Cited by 8Open Access
Abstract
Abstract Cryo-focused ion beam milling has enabled groundbreaking structural discoveries in native cells. Progress toward medically relevant applications, however, has been slow. We here present an adaptation of the cryo-lift out procedure for Serialized On-grid Lift-In Sectioning for Tomography (SOLIST), which increases throughput, reduces ice contamination, and enhances sample stability. With these improvements, new specimens, ranging from high-pressure frozen reconstituted LLPS droplets to human forebrain organoids, are accessible to cryo-electron tomography.
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