A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics
Arthur Viodé(Boston Children's Hospital), Patrick van Zalm(Boston Children's Hospital), Kinga K. Smolen(Boston Children's Hospital), Benoit Fatou(Boston Children's Hospital), David K. Stevenson(Boston Children's Hospital), Meenakshi Jha(Boston Children's Hospital), Ofer Levy(Broad Institute), Judith A. Steen(Boston Children's Hospital), Hanno Steen(Boston Children's Hospital), On behalf of the IMPACC Network
Cited by 104Open Access
Abstract
We introduce a cost-effective, robust high-throughput-compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample.
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