O-260 Rural workers and cancer (RUCAN) study a Brazilian cohort study

Abstract

<h3>Introduction</h3> The pesticides are used broadly at the worldwide and that use could cause adverse effects at health human. The RUCAN Study is the greatest Brazilian cohort aimed to assess the pesticides exposure and its correlation to cancer incidence. <h3>Material and Methods</h3> In total, 2198 participants will be sampled from Barretos and São José do Rio Preto’s regions, between 18 and 74 years old and absence of cancer history. A questionnaire containing sociodemographic information, individuals occupational and health history has been applied using REDCap software. Furthermore, blood, oral mucosa and urine samples have been collected to acetylcholinesterase determination, evaluation of the genomic instability, thought micronuclei frequency and telomere length (TL), and quantification of pesticides metabolites. The follow-ups in this cohort are expected to occur every 4 years. <h3>Results</h3> At this moment, was included 197 participants, totaling 8.96% of cohort. The main herbicide reported was glyphosate, insecticide was fipronil and fungicide was copper hydroxide. In regarding diseases, 34.0% of the participants commented be hypertensive. Furthermore, 55.5% of them showed higher total cholesterol, being that 32.3% and 24.0% of the adults until 60 years exhibited overweight and obesity, respectively. Individuals presented 92.23 ± 45.45 pb/diploid genome as mean of TL that did not show effect by gender and current pesticides exposure. On the other hand, was observed weak correlations between TL with parameters of age (r = – 0.216, p = 0.004) and pesticides exposure time (r = – 0.386, p &lt; 0.001). The quantification of metabolites of the most commonly use reported pesticides is being standardized by LC-MS/MS. <h3>Conclusions</h3> These is the preliminary results of RUCAN Study, first Brazilian cohort of rural workers. After with the complete sampling and the follow up, we hope to identify the health endpoints due pesticides exposure, as well each pesticides effects in the health endpoints.