Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
Vincent Hahaut(University of Basel), Dinko Pavlinić(University of Basel), Walter Carbone(Novartis (Switzerland)), Sven Schuierer(Novartis (Switzerland)), Pierre Balmer(University of Basel), Mathieu Quinodoz(University of Basel), Magdalena Renner(University of Basel), Guglielmo Roma(Novartis (Switzerland)), Cameron S. Cowan(University of Basel), Simone Picelli(University of Basel)
Cited by 131Open Access
Abstract
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
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